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利用从人胎肝提取的肝细胞生长刺激物质(hHss),以小鼠肝细胞质膜蛋白作为受体及内源性底物,以Poly(GluNa,Tyr)4:1作为外源性底物,通过磷酸化实验,对hHSS的作用及其与酪氨酸蛋白激酶活性之间的关系进行了研究。利用抗酪氨酸磷酸酯抗体免疫沉淀法,SDS-聚丙烯酰胺凝胶电泳和放射自显形及液体闪烁计数等方法进行测定。结果证明:hHSS可刺激并增强肝细胞膜受体的酪氨酸蛋白激酶的活性,后者可使膜蛋白某些组分及外源性底物磷酸化增强;hHSS的作用与酪氨酸蛋白激酶的活性密切相关。
Using hGas (hHss) extracted from human fetal liver, mouse hepatocyte plasma membrane protein as receptor and endogenous substrate, Poly (GluNa, Tyr) 4: 1 as exogenous substrate, By phosphorylation experiments, the role of hHSS and its relationship with tyrosine protein kinase activity were studied. Anti-tyrosine phosphate antibody immunoprecipitation, SDS-polyacrylamide gel electrophoresis and autoradiography and liquid scintillation counting and other methods were measured. The results showed that: hHSS can stimulate and enhance the activity of tyrosine protein kinase of liver cell membrane receptor, which can make certain components of membrane protein and exogenous substrate phosphorylation; hHSS role and tyrosine protein kinase The activity is closely related.