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目的:探讨脑缺血再灌注损伤后脑脊液中兴奋性氨基酸的动态变化和补阳还五汤方药对兴奋性氨基酸的影响。方法:采用微透析-高效液相色谱的方法测定大鼠脑脊液中兴奋性氨基酸的含量变化及补阳还五汤对其进行干预。结果:与假手术组相比,大鼠脑缺血后谷氨酸、天门冬氨酸在手术30～150min期间各时间观察点有显著差异(P<0.01),在手术0～15min和150～165min的观察点也有差异(P<0.05)。在手术30～120min期间各时间观察点,补阳还五汤能使缺血纹状体能使大脑纹状体内的谷氨酸显著降低(P<0.01),天门冬氨酸含量显著降低(P<0.01);在手术0～15min和120～150min再灌注的各时间观察点,补阳还五汤也能使缺血纹状体内谷氨酸含量有所降低(P<0.05)。结论:补阳还五汤使不同时间点的脑脊液中GLU、ASP含量降低,补阳还五汤抗缺血性脑损伤的保护作用机制可能与其对抗脑缺血灌注损伤后EAA的升高有关。
Objective: To investigate the dynamic changes of excitatory amino acids in cerebrospinal fluid and the effects of Buyang Huanwu Decoction on excitatory amino acids after cerebral ischemia-reperfusion injury. Methods: The levels of excitatory amino acids in rat cerebrospinal fluid (CSF) were determined by microdialysis - high performance liquid chromatography (HPLC) and the effect of Buyang Huanwu Decoction on it. Results: Compared with the sham operation group, there were significant differences in the observation points of glutamic acid and aspartic acid after 30 ~ 150min of cerebral ischemia in rats (P <0.01) There was also a difference between 165min observation points (P <0.05). During the observation period of 30 ~ 120min during operation, Buyang Huanwu decoction can make striatum of striatum significantly reduce glutamate (P <0.01) and aspartate content of striatum (P < 0.01). In the observation points of 0 ~ 15min and 120 ~ 150min reperfusion, the content of glutamic acid in the ischemic striatum of Buyang Huanwu Decoction also decreased (P <0.05). Conclusion: Buyang Huanwu Decoction can decrease the contents of GLU and ASP in cerebrospinal fluid at different time points. The protective mechanism of Buyang Huanwu Decoction on ischemic brain injury may be related to the increase of EAA after cerebral ischemia-perfusion injury.