含汞防腐剂硫柳汞作为一种抗菌剂在眼用药物和溶液中早被广泛应用。即使用比最低杀菌浓度高100倍的溶液局部滴眼,亦未发现有毒性作用。硫柳汞pH偏硷或中性,性质稳定,在4℃—38℃之间具有抗菌效能。因而许多眼内药物、冲洗液和短时角膜保存剂M-K液都用硫柳汞作抗菌防腐剂。为了观察硫柳汞对角膜内皮细胞的可能毒性作用,作者用兔和眼库中人眼角膜作体外灌注试验。用谷胱甘肽碳酸氢钠林格氏液(简称GBR) Mercury-containing preservatives Thimerosal has long been widely used as an antibacterial agent in ophthalmic drugs and solutions. Toxicity was not observed even when topical eye drops were applied 100 times higher than the minimum bactericidal concentration. Thimerosal pH alkaline or neutral, stable, with antimicrobial efficacy at 4 ℃ -38 ℃. As a result, many intraocular medications, irrigation solutions, and short-term corneal preservatives, M-K, use thimerosal as an antimicrobial preservative. In order to observe the possible toxic effects of thiomersal on corneal endothelial cells, the authors used an in vitro perfusion test with human corneas in rabbits and eye banks. With glutathione sodium bicarbonate Ringer’s solution (referred to as GBR)