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目的:探讨人参保护高皮质激素血症致神经元损伤的作用机制。方法:皮下注射皮质酮建立高皮质激素血症的动物模型,在模型的制备过程中同时ig给予高、中、低(7.2,3.6,1.8 g.kg-1)3个剂量的人参水煎剂,实验进行4周,行为学检测后处死动物,取脑剥离海马,对神经微丝蛋白(NF-L)、突触囊泡总蛋白(SYP)、脑源性神经营养因子(BDNF)、胶质源性神经营养因子(GDNF)进行蛋白免疫印迹。结果:模型组动物在行为学检测中,静止不动时间明显增加,与正常组有显著差别;体重较正常组显著降低并有显著差异。相关蛋白检测NF-L,SYP,BDNF,GDNF表达水平与正常组比较显著下调。人参高、中剂量组能够改善动物行为学变化及体重变化,人参各剂量组对神经元蛋白及神经营养因子均表现出明显的上调作用。结论:人参可通过上调神经营养因子的表达发挥神经元保护作用。
Objective: To explore the mechanism of action of ginseng in protecting cortical neurons induced by hypocortisia. METHODS: Cortisone was subcutaneously injected into cortisone to establish an animal model of hypocortisia. At the same time, three doses of high, medium and low doses (7.2, 3.6 and 1.8 g.kg-1) of ginseng water decoction After 4 weeks of experiment, animals were killed after behavioral testing. The hippocampus was dissected from the hippocampus of the brain. The expression of NF-L, SYP, BDNF, Immunohistochemistry Western blotting of neurotrophic factor (GDNF). Results: In the behavioral tests, the animals in the model group had significantly increased immobility time, which was significantly different from the normal group. The body weight of the model group decreased significantly and was significantly different. Related protein expression of NF-L, SYP, BDNF, GDNF expression was significantly lower than the normal group. The ginseng high dose group and middle dose group can improve the animal behavior change and body weight change, each dose group of ginseng has obvious up-regulation effect on neuron protein and neurotrophic factor. Conclusion: Ginseng can exert neuronal protective effect by up-regulating the expression of neurotrophic factor.