论文部分内容阅读
本研究旨在于建立DNA探针试剂盒,应用荧光原位杂交(FISH)技术检则急性髓性白血病(AML)最重要的编号染色体和结构异常。来自AML HD93多中心治疗研究组的105例病人,同时进行染色体G-显带(中期分裂相-细胞遗传学)和FISH(间期细胞遗传学)分析,并比较了两种方法的结果。结果表明,对AML的特异性染色体易位、倒位和部分三体的检测证实,间期细胞遗传学比中期细胞遗传学更敏感。本研究结论,FISH方法是染色体显带分析不失为一种有价值的补充检查。随着DNA探针的进一步发展,间期细胞遗传学可能是用于对AML的细胞遗传学分析另一方法。
The purpose of this study was to establish a DNA probe kit and use fluorescence in situ hybridization (FISH) to detect the most important numbered chromosome and structural abnormalities in acute myeloid leukemia (AML). A total of 105 patients from the AML HD93 multicenter treatment study group were simultaneously analyzed for chromosome G-banding (metaphase-cytogenetics) and FISH (interphase cytogenetics), and the results of the two methods were compared. The results showed that the detection of specific chromosomal translocations, inversions, and partial trisomics of AML demonstrated that interphase cytogenetics are more sensitive than metaphase cytogenetics. The conclusion of this study, the FISH method is a valuable supplemental examination of chromosome banding analysis. With the further development of DNA probes, interphase cytogenetics may be another method for the cytogenetic analysis of AML.