AIM:Diverse therapeutic potentials of methanolic and aqueous extracts of Oroxylum indicum(L.)Vent.bark,includ- ing antioxidant property,cytotoxicity and protection against oxidative DNA damage were investigated in this study.METHODS:Total phenolics in the extracts were determined by spectrophotometric method.Ferric reducing antioxidant property(FRAP),free radical (DPPH·and ·OH)scavenging activities,as well as inhibitory effect on lipid peroxidation have been investigated.Cytotoxicity of the extracts was investigated by XTT assay in MDA-MB-435S and Hep3B cell lines.Protection of DNA by the extracts against oxidative damage by UV-photolysis of H2O2 was studied.RESULTS:Total phenolic content and inhibition of lipid peroxidation in both extracts were found to be dosage-dependent.Moreover,both extracts exhibited considerable free radical scavenging and ferric reducing abili- ties.The extracts demonstrated extensive cytotoxicity in both tested cell lines,with the methanolic extract showing greater cytotoxic potential.Both extracts exhibited moderate levels of DNA protection against oxidative stress.Total phenolic content was noted to have significant positive correlations with free radical scavenging and reducing power,as well as lipid peroxidation inhibition(P<0.05). CONCLUSIONS:These results indicate that O.indicum bark extracts possess diverse biological activities in vitro and could render itself as a prospective candidate for drug research. AIM: Diverse therapeutic potentials of methanolic and aqueous extracts of Oroxylum indicum (L.) Vent. Bark, includ- ing antioxidant property, cytotoxicity and protection against oxidative DNA damage were investigated in this study. METHODS: Total phenolics in the extracts were determined by Spectrophotometric method. Derivative activity of extracts (FRAP), free radical (DPPH · and · OH) scavenging activities, as well as inhibitory effect on lipid peroxidation have been investigated. Cytotoxicity of the extracts by investigated by XTT assay in MDA-MB-435S and Hep3B cell lines. Prevention of DNA by the extracts against oxidative damage by UV-photolysis of H2O2 was studied .RESULTS: Total phenolic content and inhibition of lipid peroxidation in both extracts were found to be dosage-dependent. free radical scavenging and ferric reducing abili- ties. Theses extensively toxicity in both tested cell lines, with the methanolic extract showing Greater cytotoxic potential. Book extracts exhibited moderate levels of DNA protection against oxidative stress. Total phenolic content was noted to have significant positive correlations with free radical scavenging and reducing power, as well as lipid peroxidation inhibition (P <0.05). CONCLUSIONS: These results indicate that O.indicum bark extracts possessing diverse biological activities in vitro and could render itself as a prospective candidate for drug research.