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用高效液相色谱（ＨＰＬＣ）方法分析了杨树叶片磷脂酰甘油（ＰＧ）的分子种组成，用酶解和气相色谱（ＧＣ）方法分析了ＰＧ脂肪酸定位，比较了抗寒性不同的杨树（Ｐｏｐｕｌｕｓｄｅｌｔｏｉｄｅｓｃｖ．ＬｕｘＩ６９／５５和Ｐｏｅｕｒａｍｅｒｉｃａｎａｃｖ．Ｉ４５／５１）叶片ＰＧ分子种。结果表明，杨树叶片ＰＧ主要含有以下的分子种脂肪酸组成（ｓｎ１和ｓｎ２）∶１８∶３／１８∶２（１８∶２／１８∶３），１８∶３／１６∶１（３ｔ）；１８∶３／１６∶０；１８∶２／１６∶１（３ｔ）；１６∶０／１８∶２，１８∶２／１６∶０；１８∶１／１６∶１（３ｔ）；１６∶０／１６∶１（３ｔ）；１８∶１／１８∶１，１６∶０／１８∶１（１８∶１／１６∶０）；１６∶０／１６∶０。１６∶１（３ｔ）位于ｓｎ２位，１６∶０在ｓｎ１位和ｓｎ２位均有发现，Ｃ１８脂肪酸主要在ｓｎ１位，两种杨树叶ＰＧ的不饱和分子种都在７０％以上。抗寒性强的欧美杨Ⅰ４５的不饱和分子种／饱和分子种比率为３．１０，大于不抗寒的美洲黑杨Ⅰ６９的比率，杨树叶ＰＧ的饱和分子种含量之和∑［（１６∶０／１６∶０＋１６∶０／１６∶１（３ｔ）］与抗寒性密切相关，Ⅰ６９杨叶片ＰＧ的∑?
The molecular species composition of phosphatidylglycerol (PG) in poplar leaves was analyzed by high performance liquid chromatography (HPLC). The PG fatty acids were analyzed by enzymolysis and gas chromatography (GC) method. The poplar trees with different cold resistance (Populus deltoides cv.LuxI 69/55 and Poeuramericanacv.I 45/51) leaf PG molecular species. The results showed that poplar leaves PG mainly contains the following molecular species fatty acid composition (sn 1 and sn 2): 18: 3/18: 2 (18: 2/18: 3), 18: (3t); 18: 3/16: 3 (3t); 16: 0/18: 2; 18: 2/16: 16: 1/16: 16: 1 (3t); 18: 1/18: 1 16: 0/18: 1 sn 2, 16:0 in sn 1 and sn 2 were found, C18 fatty acids mainly in sn 1, two poplar leaves PG unsaturated species are more than 70%. Hardiness of Europe and the United States Yang Ⅰ 45 unsaturated molecular species / saturated molecular species ratio was 3.10, greater than the non-resistant Populus I. 69 ratio, poplar leaf PG saturated molecular species content Σ [(16:0 / 16:0 + 16:0 / 16:1 (3t)] is closely related to cold resistance, Ⅰ 69 poplar PG PG?