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目的 :探讨血清基质金属蛋白酶 - 2表达与喉癌的转移和预后的关系。方法 :采用一步酶法测定血清中 MMP-2的表达量 ,用免疫组化法检测 MMP- 2在实体瘤中的表达情况。结果 :喉癌组患者血清中 MMP- 2的表达量明显高于良性病变组和正常对照组 ,颈淋巴结转移者血清中 MMP- 2的表达水平高于非转移组 ,通过对血清中 MMP- 2的浓度和免疫组化 MMP- 2表达的灰度值进行相关分析 ,结果两者在表达程度上明显相关。结论 :检测喉癌患者血清中的 MMP- 2水平可以在术前判定肿瘤的侵袭程度和转移的可能性。
Objective: To investigate the relationship between the expression of serum matrix metalloproteinase - 2 and the metastasis and prognosis of laryngeal carcinoma. Methods: The expression of MMP-2 in serum was detected by one-step enzymatic method. The expression of MMP-2 in solid tumors was detected by immunohistochemistry. Results: The expression of MMP-2 in sera of patients with laryngeal cancer was significantly higher than that of benign lesions and normal controls. The expression of MMP-2 in serum of patients with cervical lymph node metastasis was higher than that of non-metastasis group. And the gray value of immunohistochemical MMP-2 expression were analyzed by correlation analysis. The results showed that the expression of MMP-2 and MMP-2 were significantly correlated. Conclusion: Detecting the level of MMP-2 in serum of laryngeal cancer patients can determine the degree of tumor invasion and the possibility of metastasis before surgery.