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目的:探讨干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)对人乳腺癌细胞株MCF-7的生长特性及雌激素受体(ER)亚型表达的影响。方法:以不同浓度IFN-γ(50、100和150μg/L)或IL-4(5、10和25μg/L)分别作用MCF-7细胞24、48、72和96h,MTT法检测细胞的增殖水平。选择100μg/L IFN-γ或10μg/L IL-4作用细胞96h(M/IFN-γ或M/IL-4),蛋白质印迹法检测ERα和ERβ的蛋白表达,流式细胞术分析细胞周期,RT-PCR检测凋亡抑制基因mRNA表达。结果:与对照组相比,IFN-γ作用96h后,细胞增殖活性受到抑制(100μg/L组P=0.000,150μg/L组P=0.003),G0～G1期细胞比例增多(P=0.003),S期减少(P=0.005),ERβ蛋白表达水平增加,P=0.038。IL-4作用96h后,细胞增殖活性增强(10μg/L组P=0.006,25μg/L组P=0.002),G0～G1期细胞比例减少(P=0.008),S期增多(P=0.007),ERβ蛋白表达水平减少(P=0.021),凋亡抑制基因的表达水平增加,P值分别为0.002、0.000和0.004。结论:辅助性T淋巴细胞1型(Th1)类细胞因子IFN-γ和Th2类细胞因子IL-4可分别促进或抑制ERβ表达,进而抑制或促进肿瘤细胞生长;Th1/Th2偏移对乳腺癌细胞生长特性的作用,可能与改变ER亚型表达比例有关。
Objective: To investigate the effects of interferon-γ (IFN-γ) and interleukin-4 (IL-4) on the growth and estrogen receptor (ER) subtype expression in human breast cancer cell line MCF-7. Methods: MCF-7 cells were treated with different concentrations of IFN-γ (50, 100 and 150 μg / L) or IL-4 (5, 10 and 25 μg / L) for 24,48,72 and 96 h, respectively. Level. The expression of ERα and ERβprotein was detected by Western blotting at 96h (M / IFN-γ or M / IL-4) with 100μg / L IFN-γ or 10μg / L IL-4. Cell cycle was analyzed by flow cytometry. The mRNA expression of apoptosis inhibitor was detected by RT-PCR. RESULTS: Compared with the control group, the cell proliferation activity was inhibited after IFN-γ treatment for 96h (P = 0.003 for 100μg / L group, P = 0.003 for 150μg / L group) , S phase decreased (P = 0.005), ERβ protein expression increased, P = 0.038. (P = 0.006,25μg / L, P = 0.002). The proportion of cells in G0 ~ G1 phase decreased (P = 0.008) and S phase increased (P = 0.007) , ERβprotein expression decreased (P = 0.021), apoptosis-suppressing gene expression increased, P values were 0.002,0.000 and 0.004 respectively. CONCLUSION: Th1 type cytokines IFN-γ and Th2 type cytokine IL-4 can promote or inhibit the expression of ERβ, respectively, thereby inhibiting or promoting the growth of tumor cells. Th1 / Th2 shift may play an important role in breast cancer The role of cell growth characteristics may be related to changing the expression of ER subtypes.