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碳酸酐酶(carbonic anhydrase,CA)催化CO2的可逆水合反应,是参与CO2传导而进入羧化位点的重要光合酶,其活性高低对植物水分利用及光合作用有较大影响。为了研究干旱胁迫下番茄CA表达活性变化对植株光合作用及水分利用的影响,本文利用Northern杂交检测番茄叶片的胞质CA基因在干旱胁迫下的表达;测定干旱胁迫下番茄叶片CA活性、光合速率和叶片水势变化。结果表明,番茄叶片胞质CA基因受干旱胁迫诱导;CA活性随着干旱胁迫时间延长而发生由低至高再回落的变化,且变化规律基本与CA基因相似。番茄叶片的净光合速率和水势随着干旱程度和处理时间延长而降低。在75%的土壤相对水分含量条件下,植株的CA活性、光合速率及水势变化不明显。可见番茄叶水势和光合速率与CA表达活性之间存在一定的变化趋势相似性。
Carbonic anhydrase (CA) catalyzes the reversible hydration of CO2, which is an important photosynthetic enzyme that participates in the CO2 transport and enters the carboxylation site. Its activity has a great influence on plant water use and photosynthesis. In order to study the effects of drought stress on the photosynthesis and water use efficiency of tomato plants, the expression of CA gene in tomato leaves under drought stress was detected by Northern blotting. The activity of CA, the photosynthetic rate And leaf water potential changes. The results showed that the cytoplasmic CA gene of tomato leaves was induced by drought stress. The activity of CA varied from low to high and then dropped back with the prolongation of drought stress. The variation of CA activity was basically similar to that of CA gene. The net photosynthetic rate and water potential of tomato leaves decreased with the degree of drought and treatment time. Under the condition of relative soil moisture content of 75%, the changes of CA activity, photosynthetic rate and water potential were not obvious. Visible tomato leaf water potential and photosynthetic rate and CA expression activity between the existence of certain trends similarities.