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目的 :探讨急性髓系白血病 (AML)患者化疗后粒细胞缺乏发热时血清白细胞介素 8(IL- 8)和白细胞介素 6 (IL- 6 )水平与感染的关系。方法 :采用双抗体夹心酶联免疫吸附法 (EL ISA)检测 42例 AML 患者和 2 0名正常对照者血清 IL- 8、IL- 6水平。结果 :血清 IL- 8和 IL- 6水平在 AML 患者发热第 1天均升高 ,其中病原学检查阳性组显著高于原因不明发热组 (P均 <0 .0 0 2 )和仅有临床感染证据组 (P均 <0 .0 5 ) ,仅有临床感染证据组也较原因不明发热组增高 (P均 <0 .0 2 ) ,且革兰氏阴性菌感染者较革兰氏阳性菌感染者增高明显 (P均 <0 .0 1) ;IL- 8和 IL- 6呈显著正相关 (r=0 .841,P<0 .0 0 1) ,并均与体温呈正相关 (r=0 .44 7和 0 .5 70 ,P<0 .0 0 5和 P<0 .0 0 1) ;并发败血症死亡患者的血清 IL- 8、IL- 6水平呈持续增高。结论 :应用夹心法 EL ISA联合检测血清 IL- 8、IL- 6水平 ,将有助于确定 AML 患者化疗后粒细胞缺乏发热时的早期感染及其严重性。
Objective: To investigate the relationship between serum interleukin-8 (IL-8) and interleukin-6 (IL-6) levels and infection in patients with acute myeloid leukemia (AML) after chemotherapy-induced agranulocytosis. Methods: Serum levels of IL-8 and IL-6 in 42 AML patients and 20 normal controls were detected by ELISA. Results: The levels of IL-8 and IL-6 in serum of patients with AML were all increased on the 1st day of fever, and the positive rate of etiological examination was significantly higher in patients with AML than those with unexplained fever (P <0. 02) and only clinical infection Evidence group (P <0.05), only the clinical evidence of infection than the unexplained fever group increased (P all <0. 02), and Gram-negative bacteria infection than gram-positive bacteria infection (P <0.01). There was a significant positive correlation between IL-8 and IL-6 (r = 0.841, P <0.001) .44 7 and 0 .5 70, P <0.05 and P <0.001). Serum levels of IL-8 and IL-6 in patients with septicemia were continuously increased. Conclusion: The combined detection of serum IL-8 and IL-6 levels by ELISA in sandwich ELISA will be helpful to determine the early infection and severity of agranulocytosis after chemotherapy in patients with AML.