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产肠毒素性大肠杆菌(ETEC)和霍乱弧菌均可通过定居小肠并产生肠毒素而造成急性水样腹泻。作者用转基因马铃薯表达大肠杆菌不耐热肠毒素B亚单位(LT-B)制成食用菌苗,并在小鼠中评估其可行性。作者设计并构建了编码大肠杆菌LT-B的植物可接受合成基因,将该基因插入双表达载体pTH110的烟草蚀纹病毒5’非翻译区(TEV 5’UTR)和大豆vsp 3’非翻译区之间,并由花椰菜花叶病毒35S启动子转录启
Both enterotoxigenic Escherichia coli (ETEC) and V. cholerae can cause acute watery diarrhea by colonizing the small intestine and producing enterotoxins. The authors used the potato to express Escherichia coli heat-labile enterotoxin B subunit (LT-B) to make edible bacterins and evaluated its feasibility in mice. The authors designed and constructed a plant-acceptable synthetic gene encoding E. coli LT-B, inserted it into the 5 ’untranslated region of tobacco etch virus (TEV 5’UTR) and the 3’ untranslated region of soybean vsp in the double expression vector pTH110 And transcribed from cauliflower mosaic virus 35S promoter