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用聚丙烯酰胺凝胶电泳法分析了114套杂交稻 F_1及其相应的不育系,保持系和恢复系干种子胚的酯酶同工酶,6条主要酶带分别命名为1 A、3A、4A、5A、6A 和7A。三系亲本的酯酶同工酶酶谱各有其特点。杂种 F_1的酶谱出现7种类型。当不育系具有6A 时,常与恢复系的3A 或5A 形成酶带互补的杂种,3A和6A 互补只有营养优势。5A 和6A 互补才有经济优势。本实验证明杂种 F_1酶谱的形成受亲本核质双方的影响。酯酶同工酶酶谱类型可作为预测杂种优势的生化指标之一。
114 hybrid rice F_1 and their corresponding sterile lines were analyzed by polyacrylamide gel electrophoresis. The esterase isoenzymes of maintainer line and restorer line were identified. The six major enzyme bands were named 1A, 3A , 4A, 5A, 6A and 7A. Three lines of esterase isozymes each have their own characteristics. There are 7 types of hybrid F_1. When the male sterile line has 6A, it often forms an enzyme band complementary hybrid with 3A or 5A of the restorer line, and the complementary of 3A and 6A is only a nutritional advantage. 5A and 6A complementary to have economic advantages. The experiment proves that the formation of hybrid F 1 zymogram is affected by both the parental nuclei. Esterase isozyme zymogram type can be used as one of the biochemical indicators to predict heterosis.