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蛋白激酶C（Protein kinase C,PKC）是一类丝氨酸和苏氨酸激酶,它在细胞信号传递、生长调控和肿瘤发生中具有重要作用。PKC是一个至少由12种亚类组成的多基因家族,PKC亚类的分子异质性、不同的生化性质和细胞内定位暗示其发挥不同的生理功能,为了探讨特异PKC亚类在细胞增殖、转化中的作用,我们建立了稳定过表达PKCa的人胚肺细胞模型,分析表明过表达PKCa可以促进2BS细胞的增殖速率,并能引起细胞的部分转化特征,细胞外信号一般通过细胞核内基因表达的变化最终导致细胞表型的改变,鉴于PKCa在细胞
Protein kinase C (PKC) is a class of serine and threonine kinases that play an important role in cell signaling, growth regulation and tumorigenesis. PKC is a multigene family consisting of at least 12 subtypes. The molecular heterogeneity of PKC subclasses, different biochemical properties and intracellular localization imply that it exerts different physiological functions. In order to explore the role of specific PKC subclasses in cell proliferation, We established a human embryo lung cell model stably overexpressing PKCa. The results showed that overexpression of PKCa could promote the proliferation rate of 2BS cells and induce the partial transformation of cells, and the extracellular signals were generally expressed in the nucleus by gene expression The changes eventually lead to changes in cellular phenotype, given PKCa in the cell