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目的:建立脑表面大体摄影结合图像分析的方法,观察小鼠局灶性脑缺血后24 h内血脑屏障通透性的变化。方法:采用线栓法诱导小鼠局灶性脑缺血,于缺血后10、30 m in和1、3、6、12、24 h取脑,用数码相机拍摄全脑及脑切片照片,观察脑表面和切面的出血和伊文思蓝(EB)渗出,以及血脑屏障通透性变化;并用荧光法测定脑内EB含量,以干湿重法检测脑水分含量。结果:脑表面图像分析显示,缺血后3 h开始出现明显的出血和EB渗出,与脑切片的结果一致,具有较好的相关性;荧光法显示缺血后30 m in缺血侧脑组织EB含量开始增高,缺血后1 h脑水分含量开始增加。结论:脑表面大体摄影结合图像分析,可对局灶性脑缺血后血脑屏障通透性的变化进行半定量评价,还发现脑缺血后早期血脑屏障的通透性就有增加。
OBJECTIVE: To establish a method of gross photography on the brain surface combined with image analysis to observe the changes of blood-brain barrier permeability within 24 hours after focal cerebral ischemia in mice. Methods: Focal cerebral ischemia was induced by thread occlusion. The brains were taken at 10, 30, and 1, 3, 6, 12 and 24 hours after ischemia. The whole brain and brain slices were taken with a digital camera. Brain surface and section bleeding and Evans blue (EB) exudation and changes of permeability of blood-brain barrier were observed. The content of EB in brain was measured by fluorescence method, and the content of brain water was detected by dry-wet method. Results: Brain surface image analysis showed obvious hemorrhage and EB exudation 3 h after ischemia, which was consistent with the results of brain slices. Fluorescence showed that 30 min after ischemia Tissue EB content began to increase, 1 h after ischemia brain water content began to increase. CONCLUSION: The general photography of the brain surface combined with image analysis can semi-quantitatively evaluate the changes of the permeability of the blood-brain barrier after focal cerebral ischemia. It is also found that there is an increase in the permeability of the blood-brain barrier early after cerebral ischemia.