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应用傅里叶变换红外光谱法(FTIR)对43例食管癌及癌旁正常组织进行了分析,结果表明,食管癌组织和癌旁正常组织的红外光谱具有较大的差异:正常组织的红外光谱在1550 cm-1附近的酰胺Ⅱ带吸收峰相对较强,峰型高尖,与酰胺Ⅰ带的相对峰高比(I1647/I1550)在癌组织中比较高;正常组织中酰胺Ⅰ带的吸收峰峰位比癌组织的高1-6 cm-1;正常组织中1453 cm-1处的峰强大于1402 cm-1处,而在癌组织中则相反,1402 cm-1附近的吸收峰峰高大于1453cm-1处;癌组织中1080 cm-1附近的峰位和正常组织相比显得高而强,癌组织的相对峰高比(I1080/I1550)高于正常组织。说明傅里叶变换红外光谱法可以用于对良、恶性组织进行鉴别诊断,有希望发展成为肿瘤快速诊断的一种新方法。
Forty-three cases of esophageal cancer and adjacent normal tissues were analyzed by Fourier Transform Infrared Spectroscopy (FTIR). The results showed that the infrared spectra of esophageal cancer tissues and adjacent normal tissues were quite different: the infrared spectra of normal tissues In the vicinity of 1550 cm-1, the absorption band of amide II is relatively strong, the peak shape is sharp, and the relative peak height ratio (I1647 / I1550) of amide I is relatively high in cancerous tissues; The peak-to-peak height was 1-6 cm-1 higher than that of the cancerous tissue; the peak intensity at 1453 cm-1 in normal tissue was greater than 1402 cm-1 whereas in the cancerous tissue, the absorption peak around 1402 cm-1 The peak at 1080 cm-1 in cancer tissues was higher and stronger than that in normal tissues. The relative peak height ratio (I1080 / I1550) of cancer tissues was higher than that of normal tissues. FTIR spectroscopy can be used for differential diagnosis of benign and malignant tissue, hopefully developed into a new method of rapid tumor diagnosis.