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目的研究红景天苷对慢性阻塞性肺疾病(COPD)小鼠肺损伤的保护作用及其机制研究。方法采用反复香烟烟熏法复制COPD小鼠模型。观察各组肺组织的病理学改变,测定血清白细胞介素(IL)-6、IL-1β、肿瘤坏死因子(TNF)-α和肺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的含量。Western blot检测肺组织中HO-1、Nrf-2、NF-κBp65、p-NF-κBp65、IκBα、pIκBα等氧化和炎症蛋白的表达。结果红景天苷治疗组中肺损伤明显减轻;显著降低血清中IL-6、IL-1β和TNF-α含量;增加肺组织中SOD、降低MDA含量;减少p-IκBα、p-NF-κBp65表达,增加Nrf-2、HO-1表达。结论红景天苷对慢性阻塞性肺疾病小鼠肺损伤具有保护作用,其作用可能与其调节氧化应激诱导的炎症反应相关。
Objective To study the protective effect of salidroside on lung injury in chronic obstructive pulmonary disease (COPD) mice and its mechanism. Methods Duplicate cigarette smoked COPD mouse model was duplicated. The pathological changes of lung tissue in each group were observed. The levels of serum interleukin (IL) -6, IL-1β, tumor necrosis factor (TNF) -α and malondialdehyde (MDA) and superoxide dismutase SOD) content. Western blot was used to detect the expression of HO-1, Nrf-2, NF-κBp65, p-NF-κBp65, IκBα and pIκBα in lung tissues. Results Salidroside treatment group significantly reduced lung injury; significantly decreased serum IL-6, IL-1β and TNF-α levels; increased SOD in the lung tissue, decreased MDA content; reduce p-IκBα, p-NF-κBp65 Expression, increase Nrf-2, HO-1 expression. Conclusion Salidroside has a protective effect on lung injury in mice with chronic obstructive pulmonary disease, which may be related to the regulation of oxidative stress-induced inflammation.