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为迅速扩繁 ,取红王子锦带花 (Weigelafloridacv ‘Red -Prince’)半木质化枝条 ,经消毒 ,制作成带短叶柄的茎段外植体 ,进行组培微繁研究。初始诱导较适宜的培养基为MS +BA2 0mg·L-1+NAA 0 2mg·L-1;在芽的增殖培养时 ,上述培养基中附加GA3 2 0mg·L-1可促进茎的高生长和茎的增殖数量。在嫩茎增殖培养中克服了玻璃化现象的产生
For the rapid propagation, tissue explants were dissected from the explants of the stem segments with short petioles after sterilizing the semi-lignified branches of Weigelafloridacv ’Red-Prince’. The optimum medium for initial induction was MS + BA 2 0 mg · L -1 + NAA 0 2 mg · L -1. When GA 3 20 mg · L -1 was added to the above medium, the high growth of stem And the number of stems proliferated. Vitrification phenomenon was overcome in the proliferation of young stems