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目的 改进和建立大鼠离体肝脏灌流模型。方法 采用KrebsHenseleit缓冲液(pH7.4)为灌流液基液,含2%透析48h的牛血清白蛋白组分V、20%(V∶V)洗过的人红细胞和0.3%葡萄糖。灌流速度1.5ml/(min·g),温度(37±0.5)℃,灌流压力1.07～1.33kPa。测定大鼠灌流过程中胆汁分泌量、耗氧量及灌流液pH、Na+、K+水平,并观察肝脏外观变化和组织切片的细胞形态学,评定肝脏功能。结果 本系统灌流中大鼠的各项考察指标正常,离体肝脏的存活力可达3h。结论 该模型适用于研究药物在肝脏代谢中的相互作用及其发生机制,也可用于研究某些药物特殊的代谢动力学特征
Objective To improve and establish a rat model of isolated hepatic perfusion. Methods KrebsHenseleit buffer (pH7.4) was used as perfusate base fluid, containing 2% dialyzed 48h bovine serum albumin fraction V, 20% (V: V) washed human erythrocytes and 0.3% glucose. The perfusion rate was 1.5ml / (min · g), temperature (37 ± 0.5) ℃ and perfusion pressure 1.07 ~ 1.33kPa. The bile secretion, oxygen consumption and the pH, Na + and K + of perfusate were measured during perfused rat heart. The changes of liver appearance and cell morphology were observed and the liver function was evaluated. Results The rat perfusion in various indicators of normal examination, the viability of isolated liver up to 3h. Conclusion This model is suitable for studying the interaction of drugs in hepatic metabolism and its mechanism, and it can also be used to study the special pharmacokinetic characteristics of certain drugs