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目的:探讨小儿止咳颗粒对哮喘大鼠气道高反应性的改善作用及其机制。方法:大鼠第0,8天sc及ip给予1mL含卵蛋白(OVA)100 mg的氢氧化铝凝胶和1 mL含卡介苗3 mg的生理盐水致敏,第15天开始以OVA攻击制备哮喘模型,同时ig给予小儿止咳颗粒高、中、低剂量(15.7,7.85,3.925 g·kg-1)。第22天测定大鼠气道阻力、血清中高迁移率族蛋白B-1(HMGB-1),肺匀浆中免疫球蛋白E(IgE),白介素-4(IL-4),白介素-13(IL-13)的含量。结果:小儿止咳颗粒高、中、低剂量均可明显抑制引喘后20,30,40,60 s大鼠气道所需正压力的上升(与模型组相比较P<0.01);小儿止咳颗粒各剂量组可明显抑制血清HMGB-1及肺匀浆IgE含量的增高(P<0.05,P<0.01);小儿止咳颗粒高、中剂量可以明显抑制肺匀浆IL-13,IL-4含量的增高(P<0.01)。结论:小儿止咳颗粒可以通过抑制炎症介质、细胞因子含量的增高降低哮喘大鼠的气道高反应性,从而治疗小儿上呼吸道感染后咳嗽。
Objective: To investigate the effect of Xiaozhi Zhike Granule on airway hyperresponsiveness in asthmatic rats and its mechanism. Methods: On day 0 and day 8, sc and ip rats were sensitized with 1 mL aluminum hydroxide gel containing 100 mg ovalbumin (OVA) and 1 mL saline containing 3 mg BCG. The animals were challenged with OVA on the 15th day to prepare asthma Model, while ig give children cough granules high, medium and low doses (15.7,7.85,3.925 g · kg-1). On day 22, airway resistance, high mobility group box-1 (HMGB-1), immunoglobulin E (IgE), interleukin-4 (IL- IL-13) content. Results: Xiaoer Zhike Granule could significantly inhibit the increase of positive pressure in airway of rats after 20, 30, 40 and 60 seconds of asthma induction (P <0.01 compared with the model group) Each dose group can significantly inhibit the serum HMGB-1 and lung homogenate IgE levels increased (P <0.05, P <0.01); children’s cough granules high and medium dose can significantly inhibit lung homogenate IL-13, IL-4 content Increased (P <0.01). Conclusion: Xiaozhi Zhike Granule can reduce airway hyperresponsiveness in asthmatic rats by inhibiting the increase of inflammatory mediators and cytokines, thus treating children with cough after upper respiratory tract infection.