论文部分内容阅读
采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)和荧光定量-聚合酶链式反应(Q-PCR)技术分析高体重(high weight,HW)和低体重(low weight,LW)斑点叉尾鮰鮰(Ietalurus punetaus)皮肤、鳃和胃肠道菌群多样性,为斑点叉尾鮰微生态研究及筛选斑点叉尾鮰源益生菌提供理论依据。结果显示,斑点叉尾鮰菌群丰富度由低到高依次为鳃、皮肤、前肠、后肠和胃。肠杆菌科(Enterobacteriaceae)和气单胞菌属(Aeromonas)是皮肤的优势菌群;肠杆菌科(Enterobacteriaceae)、气单胞菌属(Aeromonas)和肠球菌属(Enterococcus)是水体、鳃和胃的优势菌群;肠杆菌科(Enterobacteriaceae)、拟杆菌属(Bacteroidetes)、气单胞菌属(Aeromonas)和酵母菌属(Saccharomyces)是肠道的优势菌群。HW斑点叉尾鮰鳃菌群的香农多样性指数、均匀度和丰富度及前肠菌群的丰富度显著高于LW斑点叉尾鮰(P<0.05)。皮肤的黄杆菌属(Flavobacterium),胃的肠杆菌科(Enterobacteriaceae),前肠的拟杆菌属(Bacteroidetes)和双歧杆菌属(Bifidobacterium)及后肠的拟杆菌属(Bacteroidetes)、双歧杆菌属(Bifidobacterium)和酵母菌属(Saccharomyces)的拷贝数分别是101.97、107.69、106.19、103.83、106.13、103.92和104.26,均显著高于LW斑点叉尾鮰(P<0.05)。结果表明,斑点叉尾鮰皮肤、鳃、胃肠道均形成独特的菌群结构,LW和HW斑点叉尾鮰菌群结构存在明显差异,HW斑点叉尾鮰菌群多样性增加。
High weight (HW) and low weight (LW) were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and fluorescence quantitative polymerase chain reaction (Q-PCR) The diversity of Ietalurus punetaus skin, gill and gastrointestinal flora provides a theoretical basis for the micro-ecology of Channel catfish and the screening of catfish probiotics. The results showed that the richness of the channel catfish was gill, skin, foregut, hindgut and stomach in descending order. Enterobacteriaceae and Aeromonas are the dominant flora of the skin; Enterobacteriaceae, Aeromonas and Enterococcus are the most common bacteria in the water, gills and stomach Enterobacteriaceae, Bacteroidetes, Aeromonas and Saccharomyces are the predominant colonies of the gut. The diversity index, evenness and abundance of C. pilosa populations and the abundance of foregut flora were significantly higher than those of LW (P <0.05). Flavobacterium of the skin, Enterobacteriaceae of the stomach, Bacteroidetes and Bifidobacterium of the foregut and Bacteroidetes of the hindgut, Bifidobacterium The copy numbers of Bifidobacterium and Saccharomyces were 101.97, 107.69, 106.19, 103.83, 106.13, 103.92 and 104.26, respectively, which were significantly higher than that of LW (P <0.05). The results showed that the structures of LW and HW channel catfish were distinctly different from each other in the skin, gill and gastrointestinal tract of Channel catfish, and the diversity of the channel catfish group was increased.