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目的通过比较无创尾套法与有创颈动脉插管法测定血管紧张素原(AGT)-肾素(REN)双转基因高血压小鼠血压,分析二种血压测量方法测定转基因高血压小鼠血压的效果。方法鼠尾基因组PCR方法筛选9只雄性AGT-REN双转基因高血压小鼠,10月龄时分别采用尾套法与颈动脉插管法测定小鼠的收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)以及脉压(PP),与此同时随机选取9只相同背景的野生(WT)小鼠作为对照,对二种方法测得的各项血压值进行比较。结果对AGT-REN双转基因高血压小鼠血压测定结果显示,尾套法测定SBP、PP值均高于颈动脉插管法测得结果,而测定DBP、MAP值均低于颈动脉法测得结果,差异有统计学意义(均P<0.05);对WT小鼠血压测定结果显示,尾套法测定SBP、PP值高于颈动脉插管法测得结果,而测定DBP值低于颈动脉法测得结果,差异有统计学意义(均P<0.05),而MAP无明显差异;二种方法均显示AGT-REN双转基因小鼠各项血压值高于WT小鼠(P<0.05)。结论尾套法和有创颈动脉插管法均可监测转基因小鼠的高血压状态,但所得结果存在显著差异,应根据不同实验选取不同方法,但同一实验中二种方法不可混合使用。针对转基因高血压鼠更适合使用尾套法测量血压值。
Objective To compare the blood pressure of two hypertensive mice induced by angiotensinogen (AGT) -renin (REN) by non-invasive tail cuff method and invasive carotid artery cannulation. Two kinds of blood pressure measurement methods were used to measure the blood pressure of transgenic hypertensive mice Effect. Methods Nine male AGT-REN double-transgenic hypertensive mice were screened by tail PCR. The systolic blood pressure (SBP) and diastolic blood pressure (DBP) of mice were measured by tail cuff method and carotid artery cannulation at 10 months of age. , Mean arterial pressure (MAP) and pulse pressure (PP). At the same time, nine wild-type (WT) mice of the same background were randomly selected as controls to compare the blood pressure values measured by the two methods. Results The results of blood pressure measurement in AGT-REN double-transgenic hypertensive mice showed that SBP and PP values measured by tail cuff method were higher than those measured by carotid artery cannulation method, while DBP and MAP values were lower than those measured by carotid artery (P <0.05). The results of blood pressure measurement in WT mice showed that SBP and PP values measured by tail cuff method were higher than those measured by carotid artery cannulation method, but DBP values were lower than those of carotid arteries (P <0.05), but there was no significant difference in MAP. Both methods showed that the blood pressure of AGT-REN double transgenic mice was higher than that of WT mice (P <0.05). Conclusions Both the tail cuff method and the invasive carotid artery cannulation method can monitor the hypertension status of transgenic mice. However, the results obtained are significantly different. Different methods should be selected according to different experiments. However, the two methods can not be mixed in the same experiment. For transgenic mice more suitable for the use of tail cuff method to measure blood pressure.