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目的研究恶性间皮瘤与ｐ１６蛋白表达的关系。方法利用微波煮沸脱交联ＳＰ法，对３４例恶性间皮瘤病理标本进行了ｐ１６蛋白表达的研究。结果ｐ１６蛋白阳性表达率５３％（１８／３４），４７％（１６／３４）的病例细胞内缺乏ｐ１６蛋白的表达。多数存在ｐ１６蛋白表达的病例没有发现ｐ５３基因的点突变；反之，存在ｐ５３基因的点突变的病例多数都没有ｐ１６蛋白的表达。ｐ１６蛋白表达与ｐ５３基因点突变之间呈明显的负相关关系。结论恶性间皮瘤可能同时存在ｐ５３和ｐ１６基因的异常，肿瘤组织细胞内缺乏ｐ１６蛋白的表达可能与ｐ１６基因的纯合缺失有关。
Objective To study the relationship between malignant mesothelioma and the expression of p16 protein. Methods The expression of p16 protein in 34 cases of malignant mesothelioma was studied by microwave boiling de-crosslinking SP method. Results The positive rate of p16 protein expression was 53% (18/34), and 47% (16/34) cases lacked the expression of p16 protein. Most of the cases with p16 protein expression did not find point mutations in the p53 gene; conversely, most cases with point mutations in the p53 gene did not have p16 protein expression. There was a significant negative correlation between p16 protein expression and p53 gene point mutation. Conclusion There may be abnormal p53 and p16 genes in malignant mesothelioma at the same time. The lack of p16 protein expression in tumor tissue may be related to homozygous deletion of p16 gene.