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目的 评价中国人参对减少辐射诱导的人周围血 G0 期淋巴细胞 (PBL)微核量的作用。方法 采用细胞因子阻滞的微核法检测健康志愿者的血标本。体外 1 37Cs照射之前 ,每份血标本的单个核细胞培养以不同浓度 (0～ 2 0 0 0μg· m l- 1 )的人参根水提取物孵育 2 4 h。结果 (1)在 0 Gy时 ,每 10 0 0个双核 (BN )细胞的微核量是 11.3± 2 .7,人参水提取物≥ 5 0 0μg· ml- 1 时微核量下降 ,但微核量和人参水提取物的浓度之间的差异是显著的。 (2 )单独射线照射显著增加微核量 ,当暴露于 1Gy的照射时 ,随着培养介质中人参水提取物浓度的增加 ,微核量呈显著的线性降低 (R2 =0 .0 5 ,P =0 .0 0 2 ) ;对暴露于 1Gy辐射诱导的微核量 ,人参水提取物浓度在 15 0 0μg· m l- 1 时减少 4 6 .6 % ,浓度在 2 0 0 0μg·m l- 1时减少 5 7.6 % ;对暴露于 2 Gy辐射诱导的微核量的减少呈更强的线性相关 (R2 =0 .7,P=0 .0 0 0 1) ;(3)微核数据分析的结果呈现出与 Poisson模型相关的过度离散趋势。结果表明 :(1)人参水提取物浓度达 2 0 0 0 μg· ml- 1时 ,对 PBL 细胞毒作用无明显影响 ;(2 )人参水提取物浓度在 5 0 0 μg· ml- 1和 2 0 0 0 μg· ml- 1之间时 ,对抗 1 37Cs照射诱导的 MN发挥保护作用。结论 人参可作为一
Objective To evaluate the effect of Chinese ginseng on reducing radiation-induced micronuclei in peripheral blood G0 lymphocytes (PBL). Methods Blood samples from healthy volunteers were detected by micronucleus method with cytokine blocking. Before in vitro 1 37Cs irradiation, mononuclear cell cultures of each blood sample were incubated with different concentrations (0-200 μg·ml -1 ) of ginseng root water extract for 24 hours. Results (1) At 0 Gy, the micronucleus per 100 binuclear (BN) cells was 11.3±2.7, and the amount of micronuclei decreased when ginseng water extract was ≥500 μg·ml-1. The difference between the nucleus and the concentration of ginseng water extract is significant. (2) Radiation alone significantly increased the amount of micronuclei. When exposed to 1 Gy of irradiation, the concentration of micro-nuclei significantly decreased with the increase of the concentration of ginseng water extract in the culture medium (R2 =0.05, P). =0. 0 0 2 ) ; For exposure to 1 Gy of radiation-induced micronucleus, the concentration of ginseng water extract decreased by 46.6% at 1 500 μg·m -1 and the concentration was 2 0 0 μg·ml. - A reduction of 57.6 % at 1 hour; a stronger linear correlation with the reduction of exposure to 2 Gy radiation-induced micronucleus (R2 = 0.7, P = 0.010); (3) Micronucleus data The results of the analysis present an over-discrete trend associated with the Poisson model. The results showed that: (1) When the water extract concentration of ginseng reached 200 μg·ml-1, there was no significant effect on the cytotoxicity of PBL; (2) the concentration of ginseng water extract was 500 μg·ml-1 and At a level of 2 0 0 μg·ml-1, MN exerted protective effects against 1 37 Cs irradiation. Conclusion Ginseng can be used as a