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目的探讨脾脏动、静脉组织基质Gla蛋白(matrixGlaprotein,MGP)mRNA的表达在门静脉高压症(PHT)性血管病变中的意义。方法光镜及电镜观察对照组和PHT脾脏动、静脉病理形态学改变,采用逆转录-聚合酶链反应(RT-PCR)方法检测MGPmRNA的表达情况。结果光镜与电镜观察下与对照组脾脏血管相比,PHT组的脾脏血管均存在不同程度的平滑肌细胞增生、肥大以及与生物合成有关的细胞器增多;对照组内脾脏动、静脉组织MGPmRNA分别为(0.23±0.10)、(0.26±0.13),PHT组脾动、静脉内MGPmRNA分别为(0.58±0.19)、(0.55±0.15),对照组的显著低于PHT组,P<0.05。结论PHT血管组织MGPmRNA的表达增强,调节血管平滑肌细胞的表型转变、增殖和迁移,并参与了内脏血管病变形成和发展。
Objective To investigate the significance of matrix gravidar protein (MGP) mRNA expression in portal hypertension (PHT) -induced vascular disease in spleen. Methods The pathological changes of the arterial and venous arteries of the control group and PHT spleen were observed under light microscope and electron microscope. The expression of MGP mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Results Compared with control group, the splenic vessels in PHT group showed different degree of smooth muscle cell proliferation, hypertrophy and the number of organelles related to biosynthesis in light and electron microscopes. Compared with control group, MGP mRNA in splenic artery and vein tissue were (0.23 ± 0.10) and (0.26 ± 0.13), respectively. The splenic arterial and venous MGPmRNA in PHT group were (0.58 ± 0.19) and (0.55 ± 0.15), respectively, significantly lower than that in PHT group (P <0.05). Conclusion The expression of MGP mRNA in PHT vascular tissue is enhanced, which regulates the phenotypic changes, proliferation and migration of vascular smooth muscle cells and is involved in the formation and development of visceral vascular lesions.