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为了实现对L-半胱氨酸(L-Cys)的简便、快速检测,采用交替沉积方法(Layer-by-layer,LBL)将聚电解质PSS与多金属氧酸盐α/β-K6P2W18O62.10H2O沉积到玻碳电极上,制得[P2W18/PSS]膜修饰电极。通过循环伏安法研究该电极的电化学性质,考察L-Cys在修饰电极上的电化学行为,结果表明,该电极对L-Cys有很明显的电催化作用,L-Cys在1.4×10-4～3.8×10-3mol/L浓度范围与响应电流呈良好的线性关系,最低检测限1.0×10-5。该电极制备简单,响应快,灵敏度高,检测限低,用于检测酸奶中L-Cys含量,回收率较高。本研究结果为多金属氧酸盐应用于催化、化学分析等领域提供了理论依据。
In order to detect L-cysteine (L-Cys) easily and rapidly, the polyelectrolyte PSS was mixed with polyoxometalate α / β-K6P2W18O62.10H2O by using layer-by-layer (LBL) Deposited on the glassy carbon electrode to prepare [P2W18 / PSS] membrane modified electrode. The electrochemical properties of L-Cys on the modified electrode were investigated by cyclic voltammetry. The electrochemical behavior of L-Cys on the modified electrode was investigated. The results showed that L-Cys had an obvious electrocatalysis on L-Cys, -4 ~ 3.8 × 10-3mol / L concentration range and the response current showed a good linear relationship, the minimum detection limit of 1.0 × 10-5. The electrode has the advantages of simple preparation, fast response, high sensitivity, and low detection limit, and is used for detecting L-Cys content in yogurt with high recovery rate. The results of this study provide a theoretical basis for the application of polyoxometalates in the fields of catalysis and chemical analysis.