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目的 :探讨抑白血病因子对荷白血病鼠肝、脾组织及癌细胞超微结构的影响。方法 :纯系 6 15小鼠为实验动物 ,分三组 :a .阴性对照组 ,b .阳性对照组 ,c.治疗组 ,以抑白血病因子治疗。切除肝脾组织用戊二醛固定 ,以常规法制备电镜样品 ,透射电镜下观察三组小鼠肝、脾组织及癌细胞超微结构变化。结果 :阴性对照组肝、脾组织呈现正常结构 ,阳性对照组肝、脾组织结构异常 ,且有大量白血病细胞浸润 ,细胞超微结构以线粒体 ,内质网和核糖体变化突出。治疗组肝、脾组织结构趋于向正常转化 ,可见到脾窦内含有大量的癌细胞凋亡。结论 :抑白血病因子具有抑制白血病细胞增殖和浸润的作用。其作用机制可能与抑白血病因子促进白血病细胞分化 ,诱导白血病细胞凋亡有关
Objective: To investigate the effect of leukemia inhibitory factor on the ultrastructure of liver and spleen cells and cancer cells in leukemia-bearing mice. Methods: The pure 613 mice were divided into three groups: a. Negative control group, b. Positive control group, c. Treatment group, with leukemia factor treatment. The hepatic and splenic tissues were excised and fixed with glutaraldehyde. Electron microscopy samples were prepared by conventional method. The ultrastructural changes of liver, spleen and cancer cells were observed under transmission electron microscope. Results: The liver and spleen tissues of the negative control group showed normal structure. The positive control group had abnormal liver and spleen tissue structure, and a large number of leukemia cells infiltrated. The ultrastructure of the cells showed prominent changes in mitochondria, endoplasmic reticulum and ribosome. In the treatment group, the liver and spleen tissues tended to be transformed normally, and a large amount of apoptosis of the cancer cells was found in the splenic sinus. Conclusion: Leukemia inhibitory factor can inhibit the proliferation and invasion of leukemia cells. Its mechanism may be associated with leukemia cells to promote leukemia cell differentiation, induced apoptosis of leukemia cells