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作者用异硫氰酸胍－酚－氯仿一步法提取人脑胶质瘤总ＲＮＡ，Ｏｌｉｇｏ（ｄＴ）纤维素亲和层析分离ｐｏｌｙ（Ａ￣＋）ＲＮＡ为模板，合成的ｃＤＮＡ长度为０．２～５ｋｂ。胶质瘤ｃＤＮＡ插入片段克隆到λｇｔ１１载体，所获得的重组子在体外进行包装。该ｃＤＮＡ文库滴度为１．１２×１０￣５，克隆效率为４．８×１０￣３／ｎｇｃＤＮＡ。本文库为研究人脑胶质瘤的基因结构与功能提供了重要基础。
The authors used guanidinium isothiocyanate-phenol-chloroform to extract total RNA of human glioma by one-step method and Oligo(dT) cellulose affinity chromatography to isolate poly(A ̄+) RNA as a template. The length of synthesized cDNA was 0. 2 to 5 kb. The glioma cDNA insert was cloned into the λgt11 vector and the resulting recombinants were packaged in vitro. The titer of this cDNA library was 1.12×10 5, and the cloning efficiency was 4.8×10 3 /ng cDNA. This library provides an important basis for studying the genetic structure and function of human brain glioma.