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目的 建立氡及其子体诱发细胞转化的模型 ,模拟氡致肺癌的过程。方法 利用2 3 8PuO2 释放的α粒子 ,照射体外培养的人支气管上皮细胞系BEP2D ,诱发转化。结果 1.5Gy单次照射的R15H细胞在连续培养 2 2周后获得了转化 ,转化细胞逐步发生生长动力学和形态学的改变 ,生长失去接触抑制 ,ConA凝集现象明显 ,获得对血清诱导末端分化的抗性和锚着独立性生长能力 ,与对照细胞相比均有统计学意义上的增强。结论 从BEP2D到α粒子转化的R15H细胞能够在一定程度上模拟体内肿瘤的发生发展过程 ,可以为深入了解肺支气管肿瘤发生多阶段过程中的细胞和分子机理提供研究模型。
Objective To establish a model of radon and its progeny to induce cell transformation and simulate the process of radon-induced lung cancer. Methods The alpha particles released from 238PuO2 were irradiated to human bronchial epithelial cell line BEP2D cultured in vitro to induce transformation. Results The single-irradiated 1.5Gy R15H cells were transformed after two-and-a-half-week culture. The growth kinetics and morphological changes of the transformed cells gradually disappeared, and the growth inhibition was abolished. The ConA agglutination phenomenon was obvious. Resistance and anchorage independent growth ability, compared with the control cells were statistically significant enhancement. CONCLUSION: R15H cells transformed from BEP2D to α particles can simulate the development of tumors in vivo to some extent, and provide a research model for further understanding of the cellular and molecular mechanisms in multiple stages of pulmonary bronchial tumors.