HIV-2是引起艾滋病的主要病原之一,与HIV-1相比,其致病性弱,感染的潜伏期长,有的甚至不发展成艾滋病。但HIV-2的易感宿主较HIV-1广,除人和猩猩之外,HIV-2还能感染猴等灵长类动物,这将为艾滋病疫苗和实验动物模型的研究提供有利条件。 我们应用原核高效表达载体pBV220在大肠杆菌中分别表达了HIV-2gag全部和部分蛋白(去掉与pol重叠序列)。在此基础上我们又将gag全部和部分序列置于痘苗病毒P7.5和 HIV-2 is one of the major causes of AIDS. Compared with HIV-1, HIV-2 is less pathogenic and has longer incubation period, and some do not even develop AIDS. But HIV-2 is more susceptible to HIV-1 than HIV-1. In addition to human and orangutans, HIV-2 can infect primates such as monkeys, which will provide favorable conditions for the study of AIDS vaccine and experimental animal models. We used prokaryotic expression vector pBV220 to express all and part of the HIV-2gag protein in Escherichia coli (with the pol overlapping sequence removed). On this basis, we put all and part of the gag sequence in the vaccinia virus P7.5 and