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目的:观察血小板反应素4基因G29926C(A387P)多态性与中国苏皖地区汉族人群不稳定型心绞痛的可能关系。方法:选择2004-11/2006-05在南京医科大学第一附属医院和江苏大学附属武进医院住院不稳定型心绞痛患者110例,病例均符合2002年AHA/ACC关于不稳定型心绞痛诊断指南的诊断标准,同期选择337例非冠心病者为对照。酚-氯仿法提取白细胞DNA,应用聚合酶链反应-限制性片段长度多态性方法分析血小板反应素4A387P多态性,取部分PCR扩增产物进一步测序鉴定,比较两组间多态性频率分布差异,探讨血小板反应素4基因多态性与不稳定型心绞痛发病的可能关系。结果:447例均进入结果分析。GC基因型在不稳定型心绞痛组和对照组的分布无统计学差异(5.5%,7.1%,P=0.54),未检测到CC纯合子。C等位基因频率在不稳定型心绞痛组和对照组分别为2.7%,3.6%(P=0.55)。GC基因型与不稳定型心绞痛无显著性关联(OR=0.75,95%CI:0.30￣1.89,P=0.54)。结论:血小板反应素4基因387A→P不是中国苏皖地区汉族人群常见的多态位点,且与不稳定型心绞痛的发病无显著相关性。
Objective: To investigate the possible relationship between the polymorphism of G29926C (A387P) and the unstable angina pectoris in Han population in Jiangsu and Anhui Provinces. Methods: A total of 110 patients with unstable angina pectoris were enrolled in the First Affiliated Hospital of Nanjing Medical University and Wujin Hospital of Jiangsu University from November 2004 to May 2006. All cases were in accordance with the diagnostic criteria of AHA / ACC in 2002 for unstable angina pectoris Standard, 337 cases of non-coronary heart disease were selected as control. Phenol-chloroform method was used to extract the leucocyte DNA. Polymerase chain reaction-restriction fragment length polymorphism was used to analyze the polymorphism of 4A387P. Some PCR products were further sequenced and identified. The frequency distribution of polymorphism To explore the possible relationship between thrombospondin 4 gene polymorphism and the incidence of unstable angina pectoris. Results: All 447 cases were involved in the result analysis. GC genotype distribution in the unstable angina and control groups was not statistically different (5.5%, 7.1%, P = 0.54), CC homozygotes were not detected. The C allele frequency was 2.7% in the unstable angina group and 3.6% in the control group (P = 0.55). There was no significant association between GC genotype and unstable angina (OR = 0.75, 95% CI: 0.30-1.89, P = 0.54). CONCLUSION: Thrombopoietin 4 gene 387A → P is not a common polymorphic site in the Han population of Jiangsu and Anhui Provinces and has no significant correlation with the incidence of unstable angina pectoris.