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目的建立美罗培南在人脑脊液中浓度测定的HPLC法。方法色谱柱为C_(18)柱。流动相为乙酸胺:乙腈(90:10,V/V),紫外检测波长308nm。样品加入内标(PABA)后,乙腈直接沉淀,上清液吹干重组,进样量为20μL。结果脑脊液样品中美罗培南保留时间为3.810 min,在脑脊液药浓度0.25～50 mg·L~(-1)内线性关系良好(r=0.999 0);各浓度质控提取回收率在90%～110%之间,日内、日间RSD均<5%;-80℃冰箱保存6个月内回收率稳定。结论本研究建立的人脑脊液中美罗培南检测方法灵敏高、特异性强,适用于临床药动学的研究。
Objective To establish a HPLC method for the determination of Meropenem in human cerebrospinal fluid. Methods The column was C_ (18) column. The mobile phase was acetic acid amine: acetonitrile (90:10, V / V), UV detection wavelength 308nm. After the sample was added to the internal standard (PABA), acetonitrile was directly precipitated and the supernatant was subjected to drying and recombination. The injection volume was 20 μL. Results The retention time of meropenem in cerebrospinal fluid was 3.810 min and the linearity was good (r = 0.999 0) in the concentration range of 0.25 ~ 50 mg · L -1. The recovery of quality control was 90% ~ 110 %, Day, day RSD were <5%; - 80 ℃ refrigerator stored within 6 months the recovery rate was stable. Conclusion The detection method of Meropenem in human cerebrospinal fluid established in this study is sensitive and specific and is suitable for clinical pharmacokinetic studies.