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为了将高精度原子力显微镜(AFM)用于显示超顺磁性氧化铁标记的c-erbB2癌基因反义寡脱氧核苷酸探针(磁性反义探针)与SK-Br-3肿瘤细胞mRNA核苷酸的连接,我们在磁性反义探针转染SK-Br-3肿瘤细胞基础上,用AFM对转染后的肿瘤细胞进行观察,并同时对转染后的肿瘤细胞进行蛋白表达检测及MRI成像,以进一步证实AFM的观察结果。从AFM显示的磁性反义探针转染SK-Br-3肿瘤细胞后单个细胞的全貌图及局部放大图发现,探针中反义寡脱氧核苷酸中的脱氧胞嘧啶核苷酸闭环与肿瘤细胞mRNA嘌呤核苷酸环相连接;此外,磁性反义探针能特异性抑制SK-Br3细胞c-erbB2的蛋白表达,MRI显示磁性反义探针转染SK-Br-3肿瘤细胞的信号强度最低(P<0.05)。实验表明,AFM可以清楚显示磁性反义探针与SK-Br-3肿瘤细胞核mRNA核苷酸的连接。
In order to use a high-precision atomic force microscope (AFM) for displaying the superparamagnetic iron oxide-labeled c-erbB2 oncogene antisense oligodeoxynucleotide probe (magnetic antisense probe) and the SK-Br-3 tumor cell mRNA core In this study, we detected the transfected tumor cells by AFM on the basis of the magnetic antisense probe transfected SK-Br-3 tumor cells and detected the protein expression of the transfected tumor cells and MRI imaging to further confirm the AFM observations. From the whole cell map and the enlarged view of single cells after the magnetic antisense probe AFM showed transfection of SK-Br-3 tumor cells, it was found that the deoxycytidine nucleotides in the antisense oligodeoxynucleotides of the probe were closed-loop and In addition, magnetic antisense probe can specifically inhibit c-erbB2 protein expression in SK-Br3 cells. MRI showed that magnetic antisense probe transfected SK-Br-3 tumor cells The signal intensity was the lowest (P <0.05). Experiments show that, AFM can clearly show the magnetic antisense probe and SK-Br-3 tumor cell nucleus mRNA nucleotide connection.