论文部分内容阅读
为了探讨hMSH2mRNA及突变型P53蛋白表达与急性淋巴细胞白血病发病的关系,采用寡核苷酸探针原位杂交技术及免疫细胞化学技术检测急性淋巴细胞白血病(ALL)患者骨髓单个核细胞的滴片hMSH2mRNA和突变型P53蛋白的表达。结果显示:ALL组hMSH2mRNA表达百分比低于对照组,分别为(32.88±11.46)%和(64.22±8.51)%,两组比较有显著性差异(P<0.05);ALL组突变型P53蛋白表达百分比高于对照组,分别为(29.25±9.45)%和(12.63±6.66)%,两组比较有显著性差异(P<0.05);ALL组、对照组中hMSH2的mRNA表达百分比与突变型P53蛋白表达百分比呈负相关(r值为-0.45,P<0.05)。结论:hMSH2基因表达缺失及P53蛋白突变可能参与ALL的发生和发展;hMSH2mRNA表达缺失与突变型P53蛋白的高表达共同促进了ALL的发生。
In order to investigate the relationship between the expression of hMSH2 mRNA and mutant P53 protein and the incidence of acute lymphoblastic leukemia, oligonucleotide probe in situ hybridization and immunocytochemistry were used to detect the bone marrow mononuclear cells in patients with acute lymphoblastic leukemia (ALL) Expression of hMSH2 mRNA and mutant P53 protein. The results showed that the percentage of hMSH2 mRNA expression in ALL group was significantly lower than that in control group (32.88 ± 11.46)% and (64.22 ± 8.51)%, respectively (P <0.05). The percentage of mutant p53 protein in ALL group (29.25 ± 9.45)% and (12.63 ± 6.66)%, respectively, which were significantly different between the two groups (P <0.05). The percentage of hMSH2 mRNA expression in ALL group and control group was significantly higher than that of mutant P53 protein The percentages of expression were negatively correlated (r = -0.45, P <0.05). CONCLUSION: The loss of hMSH2 gene and the mutation of P53 may be involved in the occurrence and development of ALL. The loss of hMSH2 mRNA and the overexpression of mutant P53 protein promote the occurrence of ALL.