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目的了解广州流动儿童病毒性腹泻的感染情况。方法收集2010年5月至2011年4月广州市白云区某社区卫生服务站241例流动儿童腹泻患者的人口资料学以及粪便标本,利用酶联免疫技术(ELISA)检测轮状病毒(RV),采用逆转录聚合酶链反应(RT-PCR)法检测标本中诺如病毒(NVs)、星状病毒(AstV),聚合酶链反应(PCR)法检测腺病毒(AdV)。结果 241例患者粪便标本中RV、NVs、AdV、AstV4种病毒总检出率为56.0%(135/241),各病毒检出率分别为43.6%(105/241)、11.2%(27/241)、7.9%(19/241)、6.6%(16/241)。27株NVs阳性毒株均为GII-4;19株AdV中14株为AdV-41型,并有AdV1、2、3、31型的散在感染;AstV感染15株,均为AstV-1型。结论病毒为流动儿童腹泻的重要病因,其中轮状病毒是最主要的病原体,其他依次为NVs、AdV和AstV。
Objective To understand the prevalence of viral diarrhea in migrant children in Guangzhou. Methods The demographic data and stool samples of 241 cases of migrant children with diarrhea were collected from a community health service station in Baiyun District, Guangzhou from May 2010 to April 2011. The rotavirus (RV) was detected by enzyme-linked immunosorbent assay (ELISA) The samples were tested for the presence of norovirus (NVs), astrovirus (AstV) and polymerase chain reaction (PCR) for detection of adenovirus (AdV) by reverse transcription polymerase chain reaction (RT-PCR) Results The total detection rate of RV, NVs, AdV and AstV in 241 stool samples was 56.0% (135/241), and the detection rates of each virus were 43.6% (105/241), 11.2% (27/241) ), 7.9% (19/241), 6.6% (16/241). 27 strains of NVs were all GII-4; 14 strains of AdV-41 were AdV-41 strains and 19 strains were infected with AdV1,2,3,31; AstV-15 were all AstV-1. Conclusions The virus is an important cause of diarrhea in migrant children. Rotavirus is the most important pathogen, followed by NVs, AdV and AstV in turn.