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背景:补气健脾,补益元气,强筋健骨,疏通经络是中医对抗运动性疲劳的经典原则。目的:观察复方中药抗运动性疲劳的效应。设计:以动物为观察对象的随机对照实验。单位:浙江中医学院动物实验中心。材料:实验于2001-02/06在浙江中医学院动物实验中心完成。选用ICR小鼠80只,雌雄各半,体质量(22±2)g,SD雄性大鼠40只,体质量(194±7)g。方法:①80只小鼠随机分成两组,40只为中药健力方灌胃(实验组),40只为生理盐水灌胃(对照组)。喂养15d后,分成3组进行抗疲劳实验(n=15,游泳时间),抗缺氧能力(n=15),血红蛋白的测定(n=10)。②睾酮测定:把40只大鼠随机分成4组,生理盐水灌胃对照组、中药灌药组、运动组、运动加灌药组。灌药组的大鼠每天灌药1次,运动组及运动加灌药组每天运动1次,为3%体质量负荷游泳至力竭,水温为28℃。喂养20d后,开胸取心血2mL,离心析出血清置4℃冰箱待用,用Minigamma1275γ计数器,VCS法测定。主要观察指标:①各组小鼠的游泳时间测定。②抗缺氧时间。③血红蛋白指标及大鼠睾酮水平。结果:40只大鼠和80只小鼠均进入结果分析。①小鼠游泳时间观察:实验组较对照组明显延长[(125.2±13.7),(108.9±12.6)min,P<0.01]。②抗缺氧能力:实验组较对照组有所提高[(25.0±1.5),(23.1±1.8)min,P<0.01]。③血红蛋?
Background: Qi and spleen, replenishing vitality, strengthening gluten and strengthening bones, and clearing the meridians are the classic principles of traditional Chinese medicine to combat sports fatigue. Objective: To observe the anti-exercise fatigue effect of compound Chinese medicine. Design: Randomized controlled experiments with animals as the subject of observation. Unit: Animal Experimental Center, Zhejiang University of Traditional Chinese Medicine. MATERIALS: The experiment was performed at the Animal Experimental Center of Zhejiang College of Traditional Chinese Medicine from September 2001 to June 2006. 80 ICR mice were selected, half male and half male, body weight (22±2) g, 40 SD male rats, body weight (194±7) g. METHODS: 180 mice were randomly divided into two groups. Forty rats were intragastrically administered with Jianlifang (experimental group) and 40 rats were given normal saline (control group). After 15 days of feeding, they were divided into 3 groups for anti-fatigue test (n=15, swimming time), anti-hypoxia (n=15), and hemoglobin determination (n=10). 2 Determination of testosterone: 40 rats were randomly divided into 4 groups, normal saline gavage control group, traditional Chinese medicine irrigation group, exercise group, exercise plus irrigation group. Rats in the irrigation group were administrated once a day. The exercise group and exercise plus irrigation group were exercised once a day. They were 3% of body weight to swim until exhaustion, and the water temperature was 28°C. After 20 days of feeding, 2 mL blood was obtained by opening the chest, and the serum was collected by centrifugation at 4°C for use, and was measured with a Minigamma 1275γ counter and VCS method. MAIN OUTCOME MEASURES: 1 Determination of swimming time in each group of mice. 2 anti-anoxia time. 3 hemoglobin and rat testosterone levels. Results: Forty rats and 80 mice were involved in the result analysis. 1Mice swimming time observation: The experimental group was significantly longer than the control group [(125.2±13.7), (108.9±12.6) min, P<0.01]. 2 Anti-hypoxia: The experimental group had an increase compared to the control group [(25.0±1.5), (23.1±1.8) min, P<0.01]. 3 blood red eggs?