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目的:探讨中国苏南地区汉族人群血清C反应蛋白(CRP)水平与急性心肌梗死(AMI)发病风险及CRP基因T-757C多态性的相关性。方法:对213例AMI患者(AMI组)和173例非冠心病者(对照组)采用聚合酶链反应-限制性片段长度多态性技术检测CRP基因T-757C多态性,并采用酶联免疫吸附法检测血清CRP水平。结果:①两组患者都存在CRP基因T-757C多态性;②AMI组CRP血清水平(5.985/6.527μg/ml)与对照组(3.262/1.356μg/ml)之间差异有统计学意义(P<0.01),且经校正性别、年龄、吸烟、高血压、糖尿病和血脂异常等因素后,仍差异有统计学意义(P<0.01);③AMI组CRP基因T-757C位点CC、TC和TT 3种基因型中任意两种之间血清CRP水平均差异无统计学意义,且同性别和同年龄段亚组的该位点任何基因型之间也差异无统计学意义。结论:中国苏南地区汉族人群血清CRP水平不受CRP基因T-757C多态性的影响。
Objective: To investigate the relationship between serum C-reactive protein (CRP) levels and risk of acute myocardial infarction (AMI) and T-757C polymorphism of CRP gene in Han population of southern Jiangsu province. Methods: T-757C polymorphism of CRP gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 213 patients with AMI (AMI group) and 173 patients with non-coronary heart disease (control group) Serum CRP levels were detected by immunoadsorption assay. Results: ① The T-757C polymorphism of CRP gene existed in both groups. ② The difference between CRI serum level (5.985 / 6.527μg / ml) and control group (3.262 / 1.356μg / ml) in AMI group was statistically significant (P (P <0.01). The differences of gender, age, smoking, hypertension, diabetes and dyslipidemia were still statistically significant (P <0.01) There was no significant difference in serum CRP levels between any two genotypes in any of the three genotypes, and there was no significant difference in genotypes between any two genotypes of the same sex and age subgroups. Conclusion: Serum CRP levels in the Han population of southern Jiangsu are not affected by the polymorphism of T-757C in CRP gene.