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利用木质纤维原料生产生物乙醇作为石油的代替能源是近年来各个国家力求攻关的课题之一。在本实验中,我们选取了两个稀酸预处理条件:①170℃,30min,1:8,酸浓度1.875%;②170℃,60min,1:20,酸浓度0.75%,对玉米秸秆进行预处理,并对预处理后的固体物料进行了糖化和同步糖化发酵试验。结果表明,在糖化实验中,1号预处理条件得到的玉米秸秆其纤维素转化率为89.85%,2号预处理条件得到的玉米秸秆其纤维素转化率为81.13%。在同步同化发酵实验中,这两个预处理条件得到的玉米秸秆其最终的乙醇得率为理论值的78%和97%。说明了糖化和同步糖化发酵为两个不同的工艺阶段,其中同步糖化发酵工艺受环境的影响更多些。
The use of lignocellulosic raw materials to produce bioethanol as an alternative source of oil is one of the topics that various countries are striving to tackle in recent years. In this experiment, we selected two conditions of dilute acid pretreatment: ①170 ℃, 30min, 1: 8, acid concentration of 1.875%; ②170 ℃, 60min, 1:20, acid concentration of 0.75%, pretreatment of corn stalks The saccharification and simultaneous saccharification and fermentation of the pretreated solid material were carried out. The results showed that in the saccharification experiment, the corn stover obtained on the 1st pretreatment condition had a cellulose conversion rate of 89.85% and the corn stover obtained on the 2nd pretreatment condition had a cellulose conversion rate of 81.13%. In the simultaneous assimilation fermentation experiment, the final ethanol yield of corn stover obtained from these two pretreatment conditions was 78% and 97% of their theoretical values. It shows that saccharification and simultaneous saccharification and fermentation are two different process stages, of which simultaneous saccharification and fermentation process is more affected by the environment.