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目的：研究钙与脂多糖（ＬＰＳ）刺激大鼠腹腔巨噬细胞（ＰＭ）释放血小板活化因子（ＰＡＦ）的关系．方法：通过ＰＡＦ的生物测定法，观察了粉防己碱（Ｔｅｔ）、Ｆｕｒａ２ＡＭ和Ｂａｙｋ８６４４对ＬＰＳ刺激ＰＭ释放ＰＡＦ的影响．结果：ＬＰＳ刺激ＰＭ释放ＰＡＦ，但并不使其细胞内钙增高，Ｔｅｔ在０１，１０，１０，１００μｍｏｌ·Ｌ－１和Ｆｕｒａ２ＡＭ在００１，０１，１０，１０μｍｏｌ·Ｌ－１时降低ＬＰＳ刺激的ＰＭ释放ＰＡＦ（分别为９８±１１，６５±１６，４７±０８，３４±０４，９２±１７，５２±１３，３７±０４，３．２±０３μｇ·Ｌ－１，无药物时１１８±１２μｇ·Ｌ－１），Ｂａｙｋ８６４４在１０，５０，１０μｍｏｌ·Ｌ－１时能增加ＬＰＳ刺激的ＰＡＦ释放能增加ＬＰＳ刺激的ＰＡＦ释放（分别为１３２±１７，１６２±１４，１７６±１５μｇ·Ｌ－１），并且Ｂａｙｋ８６４４在５０μｍｏｌ·Ｌ－１时能全部或部分逆转Ｔｅｔ和Ｆｕｒａ２ＡＭ对ＰＡＦ释放的抑制作用．结论：尽管ＬＰＳ并不明显增加巨噬细胞内钙，但细胞内钙对ＬＰＳ刺激的ＰＡＦ释放是必要的．
AIM: To investigate the relationship between calcium and lipopolysaccharide (LPS) -induced release of platelet-activating factor (PAF) from rat peritoneal macrophages (PM). Methods: PAF bioassay was used to observe the effect of tetrandrine (Tet), Fura2-AM and Bayk8644 on the release of PAF by LPS-stimulated PM. Results: LPS stimulated the release of PAF by PM, but did not increase the intracellular calcium. Tet in 01, 10, 10, 100μmol·L-1 and Fura2AM in 001, 01, 1 At 10 and 10μmol·L-1, the release of PAF by LPS-stimulated PM was decreased (98 ± 11,65 ± 16,47 ± 08,34 ± 04, 92 ± 17, 52 ± 13, 37 ± 04, 3. 2 ± 03μg · L-1, 118 ± 12μg · L-1 ), Bayk8644 at 10,50,10μmol·L-1 increased LPS-stimulated PAF release can increase LPS-stimulated PAF release (132 ± 17, 162 ± 14 , 176 ± 15μg · L-1), and Bayk8644 at 5 0μmol·L-1 can be fully or partially reversed Tet and Fura2 AM inhibition of PAF release Production use. Conclusion: Although LPS does not significantly increase macrophage calcium, intracellular calcium is necessary for LPS-stimulated PAF release.