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目的研究炎症促进因子白细胞介素5（IL-5）和炎症抑制因子IL-10的变化趋势、相互关系和对炎症反应的调节作用。方法卵白蛋白腹腔注射与雾化吸入诱发BALB/c小鼠哮喘发作，动态观察哮喘动物气道炎症的改变。在激发后0、8、24、48、96h及7、14d行支气管肺泡灌洗，测定回收液中细胞总数与分类计数，酶联免疫吸附（ELISA）法测定IL-5、IL-10浓度。结果卵白蛋白激发后气道出现明显的炎症反应。8小时~7天的支气管肺泡灌洗回收液中IL-5水平的动态变化呈近似正态分布曲线，各个时相与对照组比较差异均有显著性（第7天P＜0．05，其余时相P＜0．001）。IL-10水平在8小时为（18±5）pg/ml、96小时为（19±3）mg／ml与对照组（11±4）mg/ml比较，差异有显著性（P＜0．05，P＜0.001），其余时相与对照组比较差异无显著性（P＞0．05）。嗜酸细胞计数与IL-5／IL-10比值呈密切正相关（r=0．9，P＜0．001）。结论提示IL-5和IL-10分别上行和下行调节哮喘气道炎症。
Objective To study the changes of interleukin 5 (IL-5) and interleukin-10 (IL-10), as well as the relationship between IL-5 and inflammation. Methods BALB / c mice were induced by intraperitoneal injection of ovalbumin and aerosolized inhalation. The changes of airway inflammation in asthmatic animals were dynamically observed. Bronchoalveolar lavage was performed at 0, 8, 24, 48, 96 h and 7 and 14 d after challenge. The total number of cells in the recovered fluid and the number of the cells were determined. The concentrations of IL-5 and IL-10 were measured by enzyme linked immunosorbent assay (ELISA) Results Ovalbumin-induced airway obvious inflammatory reaction. The dynamic changes of IL-5 level in the bronchoalveolar lavage recovery solution from 8 to 7 days showed a nearly normal distribution curve, with significant difference in each phase compared with the control group (P <0.05 on the 7th day and the rest Phase P <0.001). The level of IL-10 was (18 ± 5) pg / ml at 8 hours, (19 ± 3) mg / ml at 96 hours compared with that of the control group (11 ± 4) mg / ml. 05, P <0.001). There was no significant difference between the other phases and the control group (P> 0.05). There was a positive correlation between eosinophil count and IL-5 / IL-10 (r = 0.9, P <0.001). The results suggest that IL-5 and IL-10 regulate airway inflammation of the asthma up and down, respectively.