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目的 探讨亚低温 (32± 1℃ )联合黄芪对大鼠局灶脑缺血再灌注损伤的协同保护作用。方法 采用改良线栓法制备大鼠大脑中动脉闭塞再灌注模型 ,将 2 4只SD大鼠随机分为四组 (每组 6只 ) :常温组 ;亚低温组 ,再灌注后立即诱导亚低温并持续 12小时 ;黄芪组 ,脑缺血后立即按 1g/kg剂量腹腔注射黄芪 ;黄芪加亚低温组 ,脑缺血后立即按 1g/kg剂量腹腔注射黄芪 ,再灌注后立即诱导亚低温并持续 12小时。每组均脑缺血 6小时后再灌注。观察脑梗死体积、神经功能缺损评分和病理改变。结果 黄芪加亚低温组脑梗死体积为 112 .41± 16 .6 4mm3 ,亚低温组为 146 .6 8± 14.88mm3 ,经两因素方差分析有显著性意义 (P <0 .0 5 )。结论 亚低温联合黄芪对脑缺血再灌注损伤具有协同保护作用。
Objective To investigate the synergistic protective effect of mild hypothermia (32 ± 1 ℃) combined with Radix Astragali on focal cerebral ischemia-reperfusion injury in rats. Methods Rat model of middle cerebral artery occlusion and reperfusion was established by modified thread method. Twenty four SD rats were randomly divided into four groups (n = 6 each): normal temperature group, mild hypothermia group, immediately after hypoxia Astragalus group was given intraperitoneal injection of astragalus at a dose of 1g / kg, and astragalus was given intraperitoneal injection of astragalus at a dose of 1g / kg. Lasts 12 hours. Each group was reperfusion after 6 hours of cerebral ischemia. The infarct volume, neurological deficit scores and pathological changes were observed. Results The volume of cerebral infarction in astragalus plus mild hypothermia group was 112.41 ± 16.64mm3, while that in mild hypothermia group was 146.68 ± 14.88mm3. There was significant difference between the two groups (P <0.05). Conclusion Mild hypothermia combined with Astragalus has a synergistic protective effect on cerebral ischemia-reperfusion injury.