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目的探讨母体铅染毒对子一代海马组织中β淀粉样蛋白40(Aβ40)表达的影响。方法雌性小鼠自妊娠第1天开始经饮水染铅(0.3、1.0及3.0g/L,对照组饮蒸馏水)至仔鼠出生后21d断乳为止。随机抽取各组仔鼠,在出生后第7、14、21天分别测其血液和海马组织中铅的含量;在出生后第21天时,采用免疫组化方法测定各组海马组织中Aβ40的表达。结果孕哺期不同剂量铅暴露后,7、14和21天的仔鼠血铅、海马铅水平均明显高于对照组,差异有统计学意义(P<0.05)。免疫组化染色结果显示,Aβ40免疫组化阳性反应主要定位于胞浆和胞膜;各个铅暴露组仔鼠海马的CA1区域Aβ40免疫组化阳性反应物的面密度显著高于对照组,差异均有统计学意义(P<0.05);与对照组相比,各个铅暴露组仔鼠海马的CA1区域Aβ40免疫组化阳性反应物的平均灰度值明显降低,差异有统计学意义(P<0.05)。结论母体铅暴露使铅在仔鼠体内蓄积,增强了仔鼠海马组织中Aβ40的聚集,干扰了正常的海马神经元的功能,进而可能影响其学习记忆功能。
Objective To investigate the effect of maternal lead exposure on the expression of β-amyloid 40 (Aβ40) in the first generation of hippocampus. Methods Female mice were infected with lead (0.3, 1.0 and 3.0 g / L, drinking distilled water in the control group) to weaned pigs 21 days after birth on day 1 of pregnancy. The offspring of each group were randomly selected to measure the levels of lead in the blood and hippocampus on the 7th, 14th and 21st day after birth. On the 21st day after birth, the expression of Aβ40 in hippocampus of each group was determined by immunohistochemistry . Results The level of blood lead and hippocampus in the pups of 7, 14 and 21 days after exposure to different doses of lead were significantly higher than those in the control group (P <0.05). The results of immunohistochemistry showed that the positive expression of Aβ40 in the cytoplasm and membrane was mainly located in the cytoplasm and plasma membrane. The area density of Aβ40-positive immunohistochemical reagent in the hippocampus CA1 of each lead exposed group was significantly higher than that of the control group (P <0.05). Compared with the control group, the average gray value of Aβ40-positive immunohistochemical reagent in CA1 region of hippocampus in each lead exposed group was significantly lower (P <0.05) ). Conclusion Lead exposure accumulates lead in the offspring and increases the accumulation of Aβ40 in the hippocampus of the offspring, which interferes with the function of normal hippocampal neurons and may affect their learning and memory abilities.