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目的探讨活化T细胞核因子(NFAT)在慢性氟中毒大鼠氟骨症破骨细胞中的作用。方法将36只SD大鼠按体重随机分为3组(每组12只,雌雄各半):对照组(饮水含氟<0.5 mg/L)、低氟组(5.0 mg/L)、高氟组(50.0 mg/L),实验8个月后股动脉放血处死大鼠,取大鼠股骨下端,抗酒石酸酸性磷酸酶染色(TRAP)法进行破骨细胞分化鉴定;免疫组织化学法和原位杂交检测各组大鼠股骨组织中NFATc1蛋白及其mRNA表达。结果NFATc1在破骨细胞中表达阳性,与对照组[(135.90±1.03),(110.45±1.55)]比较,低氟组大鼠破骨细胞中NFATc1蛋白及mRNA[(156.81±1.26),(132.50±1.58)]表达均升高(P<0.05),高氟组大鼠破骨细胞中NFATc1蛋白及mRNA[(135.46±1.19),(110.26±1.37)]均呈下降趋势,但差异无统计学意义(P>0.05)。结论NFATc1可能是氟中毒氟骨症破骨细胞分化调节的重要环节。
Objective To investigate the role of activated T cell nuclear factor (NFAT) in osteoclasts of skeletal fluorosis in chronic fluorosis rats. Methods Thirty-six SD rats were randomly divided into three groups according to body weight: 12 in each group, half male and half in control group (drinking water containing fluorine <0.5 mg / L), low fluoride group (5.0 mg / L) (50.0 mg / L). After 8 months of experiment, the rats were sacrificed by exsanguination of the femoral artery and the lower femur of rats were sacrificed. The differentiation of osteoclasts was detected by TRAP method. Immunohistochemistry and in situ Detection of NFATc1 protein and its mRNA in the femur of rats in each group by hybridization. Results NFATc1 expression in osteoclasts was significantly higher than that in control group [(135.90 ± 1.03), (110.45 ± 1.55)], NFATc1 protein and mRNA in osteoclasts of low fluoride group [(156.81 ± 1.26), (132.50 ± 1.58)] (P <0.05). The levels of NFATc1 protein and mRNA in osteoclasts of high fluoride group [(135.46 ± 1.19) and (110.26 ± 1.37)] showed a decreasing trend, but the difference was not statistically significant Significance (P> 0.05). Conclusion NFATc1 may be an important part of the regulation of osteoclast differentiation in fluorosis with fluorosis.