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目的 :观察黄芩甙对小鼠肝细胞色素P4 50 及其亚家族的影响 ,深入探讨黄芩甙保肝作用的机理。方法 :采用分光光度法分别测定小鼠肝微粒体细胞色素P4 50 、b5含量及氨基比林N -脱甲基酶 (ADM)、7-乙氧基香豆素O -脱乙基酶 (ECD)、苯并芘羟化酶 (AHH)。采用蛋白印迹杂交技术鉴定细胞色素P4 50 同功酶。结果 :给小鼠黄芩甙 (10 0mg·kg-1·d-1)灌胃 ,连续 7d ,可使小鼠肝微粒体细胞色素P4 50 含量显著增加 ,并使ADM、ECD及AHH 3种酶活力显著增强。对 6种同功酶的鉴定结果显示 ,黄芩甙可诱导 1A1、2B1及 2C113种同功酶 ,对细胞色素b5含量及 3A2、2D1、2E13种同功酶无诱导作用。结论 :黄芩甙可显著诱导小鼠肝细胞色素P4 50 的含量和活性 ,对其亚家族的诱导以 1A1、2B1及 2C11为主。
Objective: To observe the effects of Astragalus membranaceus on hepatic cytochrome P4 50 and its subfamilies, and to further explore the mechanism of hepatoprotective effect of Astragalus membranaceus. METHODS: Spectrophotometry was used to determine the contents of cytochrome P4 50 and b5 in liver microsomes, aminopyrine N-demethylase (ADM), and 7-ethoxycoumarin O-deethylase (ECD), respectively. ), Benzoanthrone hydroxylase (AHH). Western blot hybridization was used to identify the cytochrome P4 50 isozyme. RESULTS: Gastric jaundice (100 mg·kg-1·d-1) was intragastrically administered to the mice for 7 days. The content of cytochrome P4 50 in liver microsomes of mice was significantly increased, and ADM, ECD and AHH enzymes were induced. Vitality is significantly enhanced. The identification of six isozymes showed that Astragalus can induce 1A1, 2B1 and 2C113 isoenzymes, and had no induction effect on cytochrome b5 content and 3A2, 2D1, 2E13 isozymes. Conclusion: Astragalus membranaceus could significantly induce the content and activity of hepatic cytochrome P4 50 in mice. The induction of its subfamily was mainly 1A1, 2B1 and 2C11.