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小麦条锈病是影响我国小麦生产的重要病害之一,条锈菌毒性变异是引致小麦品种抗病性丧失的主要原因。本文应用SSR分子标记技术,研究了陇南越夏区小麦条锈菌群体遗传结构,以期寻找条锈菌群体遗传重组的分子证据。研究结果表明,陇南地区小麦条锈菌群体遗传多样性比较丰富而遗传分化较小,遗传变异主要存在于群体内部,而在群体之间,遗传多样性有显著的差异。在11对SSR引物中,有4对能够检测到陇南地区小麦条锈菌群体存在遗传重组,而且重组体出现的频率不同,CPS15揭示的条锈菌重组体出现的频率为20.0%,CPS34揭示的条锈菌重组体出现的频率为18.5%,RJ20揭示的条锈菌重组体出现的频率为12.8%,RJ18揭示的条锈菌重组体出现的频率为15.0%,陇南地区小麦条锈菌群体重组体出现频率平均为16.6%。本文揭示的遗传重组现象表明陇南地区条锈菌体细胞结合十分普遍,由此推测我国小麦条锈菌在自然条件下通过遗传重组而导致毒性变异的可能性。
Wheat stripe rust is one of the important diseases that affect the wheat production in our country. The virulence variation of stripe rust is the main cause of the disease resistance loss of wheat cultivars. In this paper, SSR molecular marker technology was used to study the genetic structure of wheat stripe rust population in Longnan Yuexia District in order to find the molecular evidence of genetic recombination of the stripe rust population. The results showed that the population of wheat stripe rust in Longnan was rich in genetic diversity and less genetic differentiation, while the genetic variation mainly existed in the population. However, there were significant differences in genetic diversity among the populations. Among the 11 pairs of SSR primers, 4 pairs could detect the genetic recombination of wheat stripe rust population in Longnan area, and the frequency of recombinants was different. The frequency of stripe rust recombinant revealed by CPS15 was 20.0%. The frequency of CPS34 revealed The frequency of stripe rust recombinants was 18.5%, that of RJ8 was 12.8%, that of RJ8 was 15.0%, that of the population of wheat stripe rust in Longnan The average appearance frequency of the group was 16.6%. The phenomenon of genetic recombination disclosed in this paper shows that the combination of somatic cells in the stripe rust in Longnan is very common. Therefore, it is speculated that the possibility of virulence mutation of the wheat stripe rust in our country through genetic recombination under natural conditions.