论文部分内容阅读
目的:最新研究表明,对线粒体的分裂进行精密调控的关键蛋白是Drp1(dynamin-related protein 1,Drp1)。杏仁复合体属于中枢神经系统边缘系统的一部分,功能重要但是非常复杂。本研究拟通过荧光原位杂交(fluorescence in situ hybridization,FISH)技术对Drp1在杏仁复合体的分布特点进行描述。方法:本实验通过自行设计Drp1基因的引物、合成探针,同时采用(FISH)进行染色观察,得到Drp1 mRNA在小鼠杏仁核复合体的分布特点。结果:研究发现,Drp1 mRNA几乎分布于全部杏仁核,且在内侧杏仁核、皮质杏仁核和基底杏仁核的表达水平非常高。亚细胞水平研究进一步表明,Drp1 mRNA全部位于神经元胞体,特别是核周区域,而在突起处未见其表达。结论:以上结果提示,Drp1可能通过调节杏仁复合体处神经元的线粒体的分裂状态,参与动物的内脏活动、防御功能以及情绪变化。但是具体机制尚需进一步研究。
OBJECTIVE: Recent studies show that Drp1 (dynamin-related protein 1, Drp1) is the key protein for the precise regulation of mitochondrial fission. Almond complexes are part of the central nervous system’s limbic system and are important but complex. In this study, we will describe the distribution characteristics of Drp1 in almond complex by fluorescence in situ hybridization (FISH). Methods: In this experiment, primers of Drp1 gene were designed and synthesized. The probes were also synthesized and stained with FISH to get the distribution of Drp1 mRNA in the mouse amygdala. Results: The study found that Drp1 mRNA is distributed in almost all amygdala, and the expression level of amygdala, cortical amygdala and basal amygdala are very high. Subcellular level study further shows that, Drp1 mRNA all located in the neuronal somatic cells, especially in the perinuclear region, but no expression in the protrusion. Conclusion: The above results suggest that Drp1 may be involved in the visceral activities, defense functions and mood changes of the animals by regulating the mitochondrial division of neurons in the almond complex. However, the specific mechanism needs further study.