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目的:制备一种纳米级脂质超声微泡造影剂,观察其物理性质及稳定性,并将其运用于兔颈总动脉血栓模型,以评价其显影效果。方法:采用薄膜-水化法制备纳米级脂质微泡,分别于第1天、7天于显微镜下观察微泡形态,测量微泡粒径、表面电位,微泡浓度、物理特性。建立兔颈动脉血栓模型,分别于建模前及建模后经兔耳缘静脉团注自制纳米级脂质微泡,动态观察建模前及建模后兔颈动脉回声增强效果。结果:①显微镜下观察微泡呈球形,大小均匀,分散度良好;第1天纳米级脂质微泡平均浓度为5.0×1010个/ml,微泡粒径中位数为794.5nm,微泡粒径范围为(794.5±123.6)nm,表面电位为-9.831mV;第7天纳米脂质体微泡平均浓度为1.08×1010个/ml,微泡粒径中位数为970.8nm,微泡粒径范围为(970.8±122.5)nm,表面电位为-10.83mV。②兔颈动脉造影效果:自制纳米级脂质微泡于兔颈动脉血栓建模型前、后均能明显增加兔颈总动脉显像。结论:自制纳米级脂质微泡形态好,粒径均一,显影效果理想,为纳米颈总动脉血栓靶向性显像研究提供了研究基础。
OBJECTIVE: To prepare a nanoscale lipid ultrasound microbubble contrast medium and observe its physical properties and stability, and apply it to the rabbit common carotid artery thrombosis model to evaluate its developing effect. Methods: Nanofiber-containing lipid microbubbles were prepared by membrane-hydration method. Morphology of microbubbles were observed under the microscope on day 1 and day 7 respectively. The particle size, surface potential, microbubble concentration and physical properties were measured. The rabbit model of carotid artery thrombosis was established. The nanoscale lipid microbubbles were injected into the rabbit ear vein before and after modeling to observe dynamically the enhancement of rabbit carotid artery echo before and after modeling. Results: ① The microspheres were spherical under the microscope with uniform size and good dispersity. On the first day, the average concentration of nano-lipid microbubbles was 5.0 × 1010 cells / ml and the median diameter of the microbubbles was 794.5 nm. The average particle size range was (794.5 ± 123.6) nm and the surface potential was -9.831 mV. On the seventh day, the average concentration of nano-liposome microbubbles was 1.08 × 1010 cells / ml and the median diameter of the microbubbles was 970.8 nm. The particle size range is (970.8 ± 122.5) nm and the surface potential is -10.83mV. Rabbit carotid artery angiography: self-made nano-lipid microbubbles in rabbits before and after carotid artery thrombosis model can significantly increase the rabbit common carotid artery imaging. CONCLUSION: Self-made nanoscale lipid microbubbles have good morphology, uniform particle size and ideal developing effect, which provides the basis for the research of targeted imaging of thrombus in the common carotid artery.