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目的 观察健康成人血小板６ 种膜糖蛋白（ Ｇ Ｐ） 在不同性别、不同年龄，静息状态与活化状态时的表达特征。方法 用流式细胞仪测定静息状态下及用凝血酶受体活化肽（ Ｔ Ｒ Ａ Ｐ） 激活的血小板所结合单抗的分子数。结果 男性静息血小板和活化血小板中 Ｇ Ｐ Ⅱｂ／ Ⅲａ 和 Ｇ Ｐ Ⅲａ 显示随年龄减少现象，在静息血小板中 Ｇ Ｐ Ⅱｂ／ Ⅲａ 和 Ｇ ＰⅢａ 值与年龄呈负相关，ｒ 值分别为 － ０ ．４０９ 和－ ０ ．５３４ ，其 Ｐ 值均＜ ０ ．０５ ；而女性中未见到类似结果。 Ｔ Ｒ Ａ Ｐ 激活后 Ｇ Ｐ Ｉｂ 在血小板表面表达下降，而 Ｇ Ｐ Ⅱｂ／Ⅲａ 、Ⅲａ 、 Ｐ选择素均增高，其中 Ｐ选择素在激活后的升高值与激活前的基础值呈负相关。静息血小板的 Ｇ ＰⅠｂ 分子数约为３０ ０００ ，它与 Ｇ Ｐ Ⅴ的比值约３∶１ 。结论 健康人血小板 Ｇ Ｐ表达存在性别和年龄差异。
Objective To observe the expression characteristics of six kinds of membrane glycoproteins (G P) in healthy adults at different sexes, different ages, resting states and activated states. Methods Flow cytometry was used to determine the number of molecules bound to platelets that were activated at rest and with platelets activated by the thrombin receptor activating peptide (TRAP). Results G P Ⅱb / Ⅲa and G P Ⅲa in resting resting platelets and activated platelets showed a decrease with age. The values of G P Ⅱb / Ⅲa and G P Ⅲa in resting platelets were negatively correlated with age, with r values of -0 . 409 and - 0. 534, all P <0. 05; while women did not see similar results. The expression of GPIb on the surface of platelets decreased when activated by T R A P, while G P Ⅱb / Ⅲa, Ⅲa and P-selectin increased after activation, of which P-selectin increased after activation and the basal level before activation Negative correlation. Resting platelets G P Ⅰ b number of molecules is about 30 000, its G P Ⅴ ratio of about 3: 1. Conclusion There is a difference in gender and age in the expression of G P in healthy human.