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在肝细胞分离前5日与分离前1小时共6次经口投与小柴胡汤,其对照组投与蒸馏水。以胶原酶灌流法同时分离两组的肝细胞,进行低细胞密度培养。添加胰岛素、表皮生长因子,并在细胞内掺入~3H-胸腺嘧啶脱氧核苷,测定诱导的肝细胞DNA合成。用Williams’E培养基,根据情况添加牛胎儿血清,并探讨了变化培养时间对肝细胞再生的影响。结果,小柴胡汤组较对照组有诱
A total of 6 oral administrations of Xiaochaihu Decoction were made on the 5th day before the separation of hepatocytes and 1 hour before the separation, and the control group was administered with distilled water. Two groups of hepatocytes were simultaneously isolated by collagenase perfusion method and cultured at low cell density. Insulin, epidermal growth factor were added, and ~3H-thymidine was incorporated into the cells to determine DNA synthesis induced by hepatocytes. Bovine fetal serum was added according to the conditions using Williams’E medium, and the effect of changing culture time on hepatocyte regeneration was investigated. As a result, the Xiaochaihu Decoction group was tempted compared with the control group.