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将褐蚁、步行虫、蜈蚣的已烷、乙酸乙酯、甲醇及水提取部分和斑蝥中的斑蝥素对癌细胞株L_(121(?))、P_(33(?))和SNU-1的细胞毒作用进行了体外实验研究。 实验方法及材料:将药用昆虫室温下自然干燥、粉碎。各样品药粉舒别取50g·500ml甲醇浸泡24小时,室温下提取3次得到总提物。按常规步骤将总提物分成己烷、乙酸乙酯、甲醇和水等提取部分。另外.甲醇提取后的药物残渣用蒸馏水煮沸提取2次。每次8
Extracts of brown ant, ground beetle, terpenes hexane, ethyl acetate, methanol and water, and cantharidin in the cantharidin to cancer cell lines L_(121(?)), P_(33(?)) and SNU-1 The cytotoxicity was studied in vitro. Experimental methods and materials: The medicinal insects were naturally dried and crushed at room temperature. Each sample powder was soaked in 50 g of 500 ml of methanol for 24 hours and extracted three times at room temperature to obtain a total extract. The total extract was divided into the extraction fractions such as hexane, ethyl acetate, methanol, and water according to the conventional procedures. In addition, the drug residues after methanol extraction were extracted twice with distilled water. Every 8